{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:40:20Z","timestamp":1781530820512,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073994"],"award-info":[{"award-number":["82073994"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:p>Terpene\u2010conjugated curcuminoids are conjugates of curcuminoids and bisabolanes in the rhizomes of <jats:italic>Curcuma longa<\/jats:italic> L. The fragmentation pathways of known three terpene\u2010conjugated curcuminoids (bisabolocurcumin\u2010ether, bisabocurcumin, and demethoxybisabolocurcumin ether) and curcumin, demethoxycurcumin, and bisdemethoxycurcumin were investigated using high\u2010performance liquid chromatography\u2010electrospray ionization tandem mass spectrometry in negative mode to rapidly search and discover similar unknown compounds of the acetone fraction of turmeric. Subsequently, compounds <jats:bold>1\u20133<\/jats:bold> were founded in the acetone fraction based on molecular weight and above fragmentation pathways (the characteristic fragment ions, the most and second most abundant fragment ions produced in MS<jats:sup>2<\/jats:sup> spectra). Terpecurcumin X (<jats:bold>1<\/jats:bold>) and terpecurcumin Y (<jats:bold>3<\/jats:bold>) were further separated by liquid chromatography\u2013tandem mass spectrometry guided isolation technique to verify their structures by nuclear magnetic resonance, electrospray ionization high\u2010resolution mass spectroscopy, ultraviolet and visible spectra and infrared spectra. Interestingly, <jats:bold>1<\/jats:bold> and <jats:bold>3<\/jats:bold> were new compounds. The results indicate the feasibility and significant advantages of liquid chromatography\u2013tandem mass spectrometry for the rapid discovery and analysis of new constituents in traditional Chinese medicine. In vitro, Terpene\u2010conjugated curcuminoids had better nitric oxide inhibitory activity than the other seven curcuminoids (demethoxycurcumin, bisdemethoxycurcumin, curdione, curcumenone, bisacurone, curcumenol, and germacron).<\/jats:p>","DOI":"10.1002\/jssc.202200789","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T06:58:52Z","timestamp":1678345132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Separation and identification of terpene\u2010conjugated curcuminoids based on liquid chromatography\u2013tandem mass spectrometry and their in vitro anti\u2010inflammatory activities"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6220-3669","authenticated-orcid":false,"given":"Yangyang","family":"Liao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Southwestern Chinese Medicine Resources School of Pharmacy Chengdu University of Traditional Chinese Medicine  Chengdu P. 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