{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T07:07:18Z","timestamp":1768288038968,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:p>Taxol and 10\u2010Deacetyl baccatin III are major taxanes in the bark, needles, and endophytes of <jats:italic>Taxus baccata<\/jats:italic>. The current study aimed to develop a process for their separation from different matrices. Crude taxoid was prepared by extraction of samples with methanol, followed by partitioning with dichloromethane and precipitation with hexane. Analytical high\u2010performance liquid chromatography involved isocratic elution on C18 column (4.6 \u00d7 250\u00a0mm, 5\u00a0\u03bcm) with methanol\u2010water (70:30 v\/v) at a flow rate of 1\u00a0ml\/min. Injection volume was 20\u00a0\u03bcl and detection was carried out at 227\u00a0nm. The content of Taxol and 10\u2010Deacetyl baccatin III in bark, needles and endophytic culture broth was 11.19 and 1.75\u00a0\u03bcg\/mg; 11.19 and 1.75\u00a0\u03bcg\/mg; and 2.80 and 0.22\u00a0\u03bcg\/L, respectively. Preparative high\u2010performance liquid chromatography was done on C18 column (10 \u00d7 250\u00a0mm, 5\u00a0\u03bcm) at a flow rate of 10\u00a0ml\/min. About 20\u00a0g crude taxoid was processed in\u00a0&lt;\u00a03\u00a0h with a recovery of about 90% for both the analytes. The purity of recovered Taxol and 10\u2010Deacetyl baccatin III determined by ultra\u2010high\u2010performance liquid chromatography\u2010mass spectrometry was found to be 95.78\u00a0\u00b1\u00a03.63% and 99.72\u00a0\u00b1\u00a00.18%, respectively. The structure of recovered Taxol was confirmed by nuclear magnetic resonance. The method can find use in biotransformation studies.<\/jats:p>","DOI":"10.1002\/jssc.202200841","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T09:22:08Z","timestamp":1674638528000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Purification and characterization of Taxol and 10\u2010Deacetyl baccatin III from the bark, needles, and endophytes of <i>Taxus baccata<\/i> by preparative high\u2010performance liquid chromatography, ultra\u2010high\u2010performance liquid chromatography\u2010mass spectrometry, and nuclear magnetic resonance"],"prefix":"10.1002","volume":"46","author":[{"given":"Md.","family":"Nurullah","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zakiya","family":"Usmani","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheeraz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5110-2945","authenticated-orcid":false,"given":"Bibhu Prasad","family":"Panda","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2026-4866","authenticated-orcid":false,"given":"Saima","family":"Amin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6394-5712","authenticated-orcid":false,"given":"Showkat Rasool","family":"Mir","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02857621"},{"key":"e_1_2_9_3_1","first-page":"1150","article-title":"Himalayan yew (Taxus baccata) conservation: a vegetative approach","volume":"123","author":"Dubey KP","year":"1997","journal-title":"Indian For"},{"key":"e_1_2_9_4_1","first-page":"127","article-title":"Adventitious root formation in branch cuttings of Taxus wallichiana Zucc. (Himalayan yew): a clonal approach to conserve the scarce resource","volume":"8","author":"Aslam M","year":"2017","journal-title":"Curr Bot"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/2225-4110.136544"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-4964(15)60161-3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-1483.76418"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.12.021"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.09.005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0367-326X(00)00233-1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00738a045"},{"key":"e_1_2_9_12_1","first-page":"37","volume-title":"Taxus for taxol and taxoids","author":"Croom EM","year":"1995"},{"key":"e_1_2_9_13_1","first-page":"387","volume-title":"Preclinical and clinical studies of the taxanes","author":"Brown DT","year":"2003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00225a063"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/367630a0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8097061"},{"key":"e_1_2_9_17_1","unstructured":"ChristenAA GibsonDM BlandJ. Production of taxol or taxol\u2010like compounds in cell culture. US patent 5 019 504 publ. date May 28 1991."},{"key":"e_1_2_9_18_1","unstructured":"RamadossS VardhanA. Process for isolation of 10\u2010deacetyl baccatin\u2010III. US patent 6 124 682 publ. date September 26 2000."},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00841-9"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(01)00305-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2017.1416283"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00044a004"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)80100-I"},{"key":"e_1_2_9_24_1","unstructured":"RaoKV. Method for the isolation and purification of taxol and its natural analogues. US patent 5 475 120 publ. date December 12 1995."},{"key":"e_1_2_9_25_1","unstructured":"Bui\u2010KhacT DupuisN. Process for extraction and purification of paclitaxel from natural sources. US patent 6 452 024 B1 publ. date September 17 2002."},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800689"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826070701780672"},{"key":"e_1_2_9_28_1","first-page":"204","article-title":"Development of high performance liquid chromatography for paclitaxel purification from plant cell cultures","volume":"11","author":"Kim JH","year":"2001","journal-title":"J Microbiol Biotechnol"},{"key":"e_1_2_9_29_1","unstructured":"ICH Harmonised Tripartite Guideline.Validation of analytical procedures: text and methodology Q2(R1) International Conference on Harmonisation of Requirements for Registration of Pharmaceuticals for Human Use.Geneva;2005."},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201401030"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/pm.pm_587_19"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.1008"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations9100304"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201900754"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.0904.4041"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2021.112829"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202200841","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.202200841","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202200841","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:35:38Z","timestamp":1760873738000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202200841"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/jssc.202200841"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202200841","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2]]},"assertion":[{"value":"2022-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200841"}}