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To demonstrate the mechanism\u2010based biomarkers of Geng\u2010Nian\u2010Shu in treating perimenopausal syndrome, a total of 135 chemical constituents including 52 prototype blood constituents were identified via high\u2010performance liquid chromatography\u2010quadrupole\u2010time of flight\/mass spectrometry. Then, network pharmacology showed significant enrichment for the PhosphoInositide\u20103 Kinase\/Akt pathway, suggesting that it may be the main regulatory pathway for the Geng\u2010Nian\u2010Shu treatment of the perimenopausal syndrome. Subsequently, multivariate analysis was performed between the Geng\u2010Nian\u2010Shu sham\u2010treated and Geng\u2010Nian\u2010Shu ovariectomy\u2010treated groups and further screened out 18 prototype blood constituents by correlation analysis with plasma estrogen levels to identify potential biomarkers associated with Geng\u2010Nian\u2010Shu treat the ovariectomy\u2010induced perimenopausal syndrome. Finally, the results of pharmacological experimental verification and Pearson correlation analysis indicated that catalpol, ligustilide, paeoniflorin, and gallic acid were selected as biomarkers of Geng\u2010Nian\u2010Shu which were strongly and positively correlated with PhosphoInositide\u20103 Kinase\/Akt signaling pathway. In this study, based on high\u2010performance liquid chromatography\u2010quadrupole\u2010time of flight\/mass spectrometry combined with pharmacodynamics, network pharmacology, pharmacology, and other disciplines, we explored the effects and mechanisms of Geng\u2010Nian\u2010Shu in the treatment of perimenopausal syndrome at multiple levels. Using multiplatform technology to investigate the role of Geng\u2010Nian\u2010Shu represents a new strategy for the selection and verification of biomarkers of Geng\u2010Nian\u2010Shu and provides a basis for further development and utilization of Geng\u2010Nian\u2010Shu.<\/jats:p>","DOI":"10.1002\/jssc.202300011","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T05:20:06Z","timestamp":1687584006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Biomarkers based on multiplatform comprehensive analysis: A systematic analysis of Geng\u2010Nian\u2010Shu in perimenopausal syndrome"],"prefix":"10.1002","volume":"46","author":[{"given":"Xinyan","family":"Lv","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University  Shenyang Liaoning P.\u00a0R.\u00a0China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghua","family":"Xu","sequence":"additional","affiliation":[{"name":"Faculty of Functional Food and Wine Shenyang Pharmaceutical University  Shenyang Liaoning P. 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