{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:17:34Z","timestamp":1769127454818,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200311"],"award-info":[{"award-number":["32200311"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MH396"],"award-info":[{"award-number":["ZR2020MH396"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>In this work, the preparative separation of quinolyridine alkaloids from seeds of <jats:italic>T. lanceolata<\/jats:italic> by conventional and pH\u2010zone\u2010refining counter\u2010current chromatography. Traditional counter\u2010current chromatography separation was performed by a flow\u2010rate changing strategy with a solvent system of ethyl acetate\u2010<jats:italic>n<\/jats:italic>\u2010butanol\u2010water (1:9:10, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) and 200\u00a0mg sample loading. Meanwhile, the pH\u2010zone\u2010refining mode was adopted for separating 2.0\u00a0g crude alkaloid extracts with the chloroform\u2010methanol\u2010water (4:3:3, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) solvent system using the stationary and mobile phases of 40\u00a0mM hydrochloric acid and 10\u00a0mM triethylamine. Finally, six compounds, including <jats:italic>N<\/jats:italic>\u2010formylcytisine (two conformers) (<jats:bold>1<\/jats:bold>), <jats:italic>N<\/jats:italic>\u2010acetycytisine (two conformers) (<jats:bold>2<\/jats:bold>), (\u2010)\u2010cytisine (<jats:bold>3<\/jats:bold>), 13\u2010<jats:italic>\u03b2<\/jats:italic>\u2010hydroxylthermopsine (<jats:bold>4<\/jats:bold>), <jats:italic>N<\/jats:italic>\u2010methylcytisine (<jats:bold>5<\/jats:bold>), and thermopsine (<jats:bold>6<\/jats:bold>) were successfully obtained in the two counter\u2010current chromatography modes with the purities over 96.5%. Moreover, we adopted nuclear magnetic resonance and mass spectrometry for structural characterization. Based on the obtained findings, the pH\u2010zone\u2010refining mode was the efficient method to separate quinolyridine alkaloids relative to the traditional mode.<\/jats:p>","DOI":"10.1002\/jssc.202300053","type":"journal-article","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T02:39:37Z","timestamp":1687919977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Separation and purification of quinolyridine alkaloids from seeds of <i>Thermopsis lanceolata<\/i> R. Br. by conventional and pH\u2010zone\u2010refining counter\u2010current chromatography"],"prefix":"10.1002","volume":"46","author":[{"given":"Fansheng","family":"Ning","sequence":"first","affiliation":[{"name":"Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. R. 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