{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T09:03:27Z","timestamp":1789635807120,"version":"4.0.0"},"reference-count":28,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>\n                    It has been proved that purine metabolites are implicated in various biological syndromes and disorders. Therefore, the realization of panoramic detection of purine metabolites will be of great significance to the pathogenesis of purine metabolic disorders. In the present study, an ultra\u2010high performance liquid chromatography with tandem mass spectrometry method was developed for the comprehensive quantification of purine metabolites in rat plasma. The 17 purine metabolites were separated and quantified in the short running time of 15\u00a0min. The proposed method was strictly validated by applying SeraSub solution as a matrix and proved to be linear (R\n                    <jats:sup>2<\/jats:sup>\n                    \u2265 0.9944), accurate (the recoveries of all analytes ranged from 85.3% to 103.0%, with relative standard deviation values \u2264 9.3%), and precise (the intra\u2010 and inter\u2010day precisions were less than 10.8% and 12.4%, respectively). The method was then successfully applied to the qualification of the endogenous purine metabolites in acute gouty arthritis rats, as well as colchicine and anthocyanin\u2010intervened rats. Results showed that uric acid, xanthine, hypoxanthine, and xanthine were considered the key factors of acute gouty arthritis. The established method and measurement of purines in rat plasma might help the investigation of the action mechanisms between purine disorders and related diseases.\n                  <\/jats:p>","DOI":"10.1002\/jssc.202300090","type":"journal-article","created":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T01:56:00Z","timestamp":1694224560000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A liquid chromatography\u2010tandem mass spectrometry method for comprehensive determination of metabolites in the purine pathway of rat plasma and its application in anti\u2010gout effects of\n                    <i>Lycium ruthenicum<\/i>\n                    Murr."],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-5479","authenticated-orcid":false,"given":"Qiangqiang","family":"Jia","sequence":"first","affiliation":[{"name":"Analysis and Test Center\uff0c State Key Laboratory of Plateau Ecology and Agriculture Qinghai University  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zufan","family":"Yang","sequence":"additional","affiliation":[{"name":"Analysis and Test Center\uff0c State Key Laboratory of Plateau Ecology and Agriculture Qinghai University  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Analysis and Test Center\uff0c State Key Laboratory of Plateau Ecology and Agriculture Qinghai University  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haishan","family":"Yang","sequence":"additional","affiliation":[{"name":"Analysis and Test Center\uff0c State Key Laboratory of Plateau Ecology and Agriculture Qinghai University  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Functional Materials Chemistry\uff0c School of Chemistry and Molecular Engineering East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Functional Materials Chemistry\uff0c School of Chemistry and Molecular Engineering East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Jiujiang First People's Hospital Affiliated Jiujiang Hospital of Nanchang University  Jiujiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Yan'an University Affiliated Hospital  Yan'An 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