{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T14:18:36Z","timestamp":1785766716202,"version":"3.56.0"},"reference-count":50,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:p>The inhibition of tyrosinase is considered to be a common therapeutic strategy for some hyperpigmentation disorders. Screening of tyrosinase inhibitors is of great significance to the treatment of pigmentation diseases. In this study, tyrosinase was covalently immobilized on magnetic multi\u2010walled carbon nanotubes for the first time, and the immobilized tyrosinase was applied for ligand fishing of tyrosinase inhibitors from complex medicinal plants. The immobilized tyrosinase was characterized by transmission electron microscopy, atomic force microscopy, Fourier\u2010transform infrared spectroscopy, vibrating\u00a0sample\u00a0magnetometry, and thermo\u2010gravimetric analyzer, which indicated that tyrosinase was immobilized onto magnetic multi\u2010walled carbon nanotubes. The immobilized tyrosinase showed better thermal stability and reusability than the free one. The ligand was fished out from Radix Paeoniae Alba and identified as 1,2,3,4,6\u2010pentagalloylglucose by ultra\u2010performance liquid chromatography\u2010quadrupole time\u2010of\u2010flight high\u2010resolution mass spectrometry. 1,2,3,4,6\u2010pentagalloylglucose was found to be a tyrosinase inhibitor with similar half maximal inhibitory concentration values of 57.13\u00a0\u00b1\u00a00.91\u00a0\u03bcM compared to kojic acid (41.96\u00a0\u00b1\u00a00.78\u00a0\u03bcM). This work not only established a new method for screening tyrosinase inhibitors but also holds considerable potential for exploring the new medicinal value of medicinal plants.<\/jats:p>","DOI":"10.1002\/jssc.202300195","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T04:59:06Z","timestamp":1685077146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Covalent immobilization of tyrosinase on magnetic multi\u2010walled carbon nanotubes for inhibitor screening"],"prefix":"10.1002","volume":"46","author":[{"given":"Yue","family":"Li","sequence":"first","affiliation":[{"name":"School of Science Xihua University  Chengdu P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Xin","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Science Xihua University  Chengdu P. R. 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