{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:11:04Z","timestamp":1770340264177,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T00:00:00Z","timestamp":1695340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>In this work, eight compounds from <jats:italic>Phellodendron chinense<\/jats:italic> were separated and purified by pH\u2010zone refining counter\u2010current chromatography and traditional counter\u2010current chromatography coupled with online\u2010storage inner\u2010recycling counter\u2010current chromatography (IRCCC). The pH\u2010zone\u2010refining mode was adopted for separating 2.0\u00a0g of crude extract with the solvent system of chloroform\u2013methanol\u2013water (4:3:3, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>), in which 10\u00a0mM hydrochloric acid and 10\u00a0mM triethylamine were added in the stationary and mobile phases, respectively. Meanwhile, traditional counter\u2010current chromatography coupled with online\u2010storage IRCCC separation was performed by the solvent system of <jats:italic>n<\/jats:italic>\u2010hexane\u2010ethyl acetate\u2010methanol\u2010water (5:5:2:8, <jats:italic>v\/v<\/jats:italic>). Finally, eight compounds, including six alkaloids as 6\u2010methylpiperidin\u20102\u2010one(<jats:bold>1<\/jats:bold>), isoplatydesmine(<jats:bold>4<\/jats:bold>), berlambine(<jats:bold>5<\/jats:bold>), epiberberine(<jats:bold>6<\/jats:bold>), palmatine(<jats:bold>7<\/jats:bold>), berberine(<jats:bold>8<\/jats:bold>) and two phenolic acids as ferulic acid(<jats:bold>2<\/jats:bold>), isoferulic acid(<jats:bold>3<\/jats:bold>), were successfully obtained using these three different CCC modes with the purities over 95.0%.<\/jats:p>","DOI":"10.1002\/jssc.202300497","type":"journal-article","created":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T08:53:31Z","timestamp":1695372811000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Separation and purification of alkaloids and phenolic acids from <i>Phellodendron chinense<\/i> by pH\u2010zone refining and online\u2010storage inner\u2010recycling counter\u2010current chromatography"],"prefix":"10.1002","volume":"46","author":[{"given":"Heng","family":"Zhu","sequence":"first","affiliation":[{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan P. 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