{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:43:29Z","timestamp":1791391409243,"version":"4.3.3"},"reference-count":37,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:p>In the present study, polysaccharide\u2010based columns were used to evaluate the efficiency of columns in response to the introduction of water and protic solvents (methanol and ethanol) into the mobile phase, replacing acetonitrile. While increasing water content frequently enhances enantiomer resolution, the inclusion of water, particularly when combined with methanol and ethanol in the mobile phase, has an adverse impact on mass transfer, thus influencing the column plate height. These effects are more pronounced with ethanol, and in many cases, van Deemter plots exhibit the absence of a minimum point optimal in the explored range. Consequently, acetonitrile and its water mixtures are the preferred choices to mitigate these effects for situations in which the chiral column is operated at a relatively high flow rate\u00a0(&gt;\u00a01\u00a0mL\/min in a 4.6\u00a0mm column).<\/jats:p>","DOI":"10.1002\/jssc.202300538","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T07:20:33Z","timestamp":1698391233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of water and protic solvents on polysaccharide\u2010based column efficiency"],"prefix":"10.1002","volume":"46","author":[{"given":"Alberto","family":"dos Santos Pereira","sequence":"first","affiliation":[{"name":"Department of Anlytical Chemistry Paraza Pharma Inc.  Saint\u2010Laurent Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25081931"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00330a057"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0693823"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00743-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00648-3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500515"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1246\/cl.1989.1089"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-100101505"},{"key":"e_1_2_9_10_1","first-page":"425","article-title":"Evaluation of Performance of commercially available polysaccharide\u2010based chiral stationary phases after multicycle operation in multimodal elution mode","volume":"39","author":"T\u00f6r\u00f6k G","year":"2005","journal-title":"J Liq Chromatogr Related Technol"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00706-021-02832-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2018.04.017"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00955-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.06.040"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2021.116332"},{"key":"e_1_2_9_16_1","article-title":"Evaluation of a polysaccharide\u2010based chiral reversed\u2010phase liquid chromatography screen strategy in pharmaceutical analysis","volume":"1672","author":"Lin Z","year":"2021","journal-title":"J Chromatogr A"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201800696"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(94)80400-1"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00719-5"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201200443"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2022.339608"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900791"},{"key":"e_1_2_9_23_1","first-page":"1006","article-title":"A simple instrumental approach for \u2018supercritical\u2019 fluid chromatography in drug discovery and its consequences for coupling with mass spectrometric and light scattering detection","volume":"29","author":"Brunelli C","year":"2011","journal-title":"LCGC North Am"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2019.115648"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03908"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9GC04207E"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2021.116464"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113227"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/15422119.2022.2038625"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)00746-X"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500372"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.07.092"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00706-020-02622-5"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.04.055"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.09.024"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.01.040"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22559"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.11.049"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202300538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:38:06Z","timestamp":1760866686000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202300538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,27]]},"references-count":37,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/jssc.202300538"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202300538","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,27]]},"assertion":[{"value":"2023-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300538"}}