{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:37:05Z","timestamp":1776386225959,"version":"3.51.2"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973471"],"award-info":[{"award-number":["81973471"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274067"],"award-info":[{"award-number":["82274067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Pseudoallergy is a typical and common adverse drug reaction to injections, especially in traditional Chinese medicine injections (TCMIs). At present, the evaluation methods for pseudoallergy include cell methods in vitro and animal methods in vivo. The mast cell evaluation method based on the \u03b2\u2010hexosaminidase (\u03b2\u2010Hex)\u2010catalyzed substrate, 4\u2010nitrophenyl\u2010\u03b2\u2010N\u2010acetyl\u2010D\u2010glucosaminide (4\u2010NPG), is an important method for the evaluation of drug\u2010induced pseudoallergy, but it is prone to false positive results and has insufficient sensitivity. In this study, a novel \u03b2\u2010Hex evaluation system with rat basophilic leukemia\u20102H3 cells based on high\u2010performance liquid chromatography\u2010fluorescence detection (HPLC\u2010FLD) was established, which effectively increased the sensitivity and avoided false positive results. Cell viabilities were measured by 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl tetrazolium bromide assay. In addition, a method for the determination of histamine, which is another indicator in the development of pseudoallergy, was established to validate the above method. The results of this novel method indicated that two TCMIs (Shuxuening injection and Shenqi Fuzheng injection), which were considered to be pseudoallergenic using 4\u2010NPG, were not pseudoallergenic. Overall, the novel \u03b2\u2010Hex\/HPLC\u2010FLD evaluation system using Rat basophilic leukemia\u20102H3 cells established was effective and precise. It could be used for the evaluation of pseudoallergic reactions caused by TCMIs and other injections.<\/jats:p>","DOI":"10.1002\/jssc.202300545","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:01:45Z","timestamp":1705017705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel method for evaluating pseudoallergy based on \u03b2\u2010hexosaminidase and its application for traditional Chinese medicine injections"],"prefix":"10.1002","volume":"47","author":[{"given":"Chen","family":"Yang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of TCM Evaluation and Translational Research School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of TCM Evaluation and Translational Research School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7493-1906","authenticated-orcid":false,"given":"Jin","family":"Qi","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of TCM Evaluation and Translational Research School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1401-9250","authenticated-orcid":false,"given":"Bo\u2010Yang","family":"Yu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of TCM Evaluation and Translational Research School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"54","article-title":"Nonallergic hypersensitivity to nonsteroidal antiinflammatory drugs, angiotensin\u2010converting enzyme inhibitors, radiocontrast media, local anesthetics, volume substitutes and medications used in general anesthesia","volume":"17","author":"Toncic RJ","year":"2009","journal-title":"Acta Dermatovenerol Croat"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0254-6272(13)60085-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pds.4805"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201400678"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108389"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000479878"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201400280"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2003.09.010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fshw.2018.10.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00208-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04758"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03580-5"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105457200"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4672"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2020.1784238"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf980901f"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.12.041"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.09.028"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7RA11507E"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac030355b"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3358\/shokueishi.59.121"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3358\/shokueishi.53.121"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5RA24301G"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200326050-00001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2004.03.017"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-005-2975-7"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.12674"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.06.008"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi201284u"},{"key":"e_1_2_9_31_1","first-page":"333","article-title":"Die Kinetik der Invirtinwirkung","volume":"49","author":"Michaelis L","year":"1913","journal-title":"Biochem Z"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202300545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:48:24Z","timestamp":1760881704000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202300545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/jssc.202300545"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202300545","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300545"}}