{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:08Z","timestamp":1786978088164,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T00:00:00Z","timestamp":1696550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 112\u20102113\u2010M\u2010017\u2010004"],"award-info":[{"award-number":["NSTC 112\u20102113\u2010M\u2010017\u2010004"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 112\u20102113\u2010M\u2010143\u2010004"],"award-info":[{"award-number":["NSTC 112\u20102113\u2010M\u2010143\u2010004"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:p>\n                    This study developed a facile, highly sensitive technique for extracting and quantifying barbiturates in serum samples. This method combined ultrasound and surfactant\u2010assisted dispersive liquid\u2013liquid microextraction with poly(ethylene oxide)\u2010mediated stacking in capillary electrophoresis. Factors influencing the extraction and stacking performance, such as the type and volume of extraction solvents, the type and concentration of surfactant, extraction time, salt additives, sample matrix, solution pH, and composition of the background electrolyte, were carefully studied and optimized to achieve the optimal detection sensitivity. Under the optimized extraction (injecting 140\u00a0\u03bcL C\n                    <jats:sub>2<\/jats:sub>\n                    H\n                    <jats:sub>4<\/jats:sub>\n                    Cl\n                    <jats:sub>2<\/jats:sub>\n                    into 1\u00a0mL of sample with pH 4 (5\u00a0mM sodium phosphate containing 0.05\u00a0mM Tween 20 and sonication for 1\u00a0min) and separation conditions (150\u00a0mM tris(hydroxymethyl)aminomethane\u2010borate with pH 8.5 containing 0.5% (m\/v) poly(ethylene oxide)), the limits of detection (signal\u2010to\u2010noise ratio\u00a0=\u00a03) of five barbiturates ranged from 0.20 to 0.33\u00a0ng\/mL, and the calculated sensitivity improvement ranged from 868\u2010 to 1700\u2010fold. The experimental results revealed excellent linearity (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0&gt;\u00a00.99), with relative standard deviations of 2.1%\u20133.4% for the migration time and 4.3%\u20135.7% for the peak area. The recoveries of the spiked serum samples were 97.1% \u2013110.3%. Our proposed approach offers a rapid and practical method for quantifying barbiturates in biological fluids.\n                  <\/jats:p>","DOI":"10.1002\/jssc.202300557","type":"journal-article","created":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T14:34:21Z","timestamp":1696775661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ultrasound and surfactant\u2010assisted dispersive liquid\u2013liquid microextraction prior to poly(ethylene oxide)\u2010mediated stacking in CE for highly sensitive determination of barbiturates in human fluids"],"prefix":"10.1002","volume":"46","author":[{"given":"Li Hsin","family":"Tseng","sequence":"first","affiliation":[{"name":"Department of Chemistry National Kaohsiung Normal University  Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Chi","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Chemistry 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