{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:41:22Z","timestamp":1784263282220,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Loxoprofen sodium is a chiral drug with two chiral centers. In our previous work, we found that the elimination of its four isomers showed stereospecificity in rats, while how the stereospecific behavior occurred in vivo was unclear. To clarify this issue, each single isomer of loxoprofen sodium was prepared by a chiral semi\u2010preparative high\u2010performance liquid chromatography (HPLC) and then administered to rats. By analysis of each isomer in rat plasma utilizing an analytical chiral HPLC, it was discovered that the chiral inversion occurred only to its (2<jats:italic>R<\/jats:italic>)\u2010isomers, one from (1\u2032<jats:italic>S<\/jats:italic>,2<jats:italic>R<\/jats:italic>)\u2010 to (1\u2032<jats:italic>S<\/jats:italic>,2<jats:italic>S<\/jats:italic>)\u2010isomer and the other from (1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>R<\/jats:italic>)\u2010 to (1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>S<\/jats:italic>)\u2010isomer. The reduction of \u03b1\u2010substituted cyclopentanone occurred only to its (1\u2032<jats:italic>R<\/jats:italic>)\u2010isomers, with (1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>R<\/jats:italic>)\u2010isomer reduced to (2\u2032<jats:italic>S<\/jats:italic>,1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>R<\/jats:italic>)\u2010<jats:italic>trans<\/jats:italic>\u2010alcohol and (1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>S<\/jats:italic>)\u2010 to (2\u2032<jats:italic>S<\/jats:italic>,1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>S<\/jats:italic>)\u2010<jats:italic>trans<\/jats:italic>\u2010alcohol. Interestingly, both the inversion and the reduction reaction occurred to its (1\u2032<jats:italic>R<\/jats:italic>,2<jats:italic>R<\/jats:italic>)\u2010isomer due to the special stereo\u2010structure with both (2<jats:italic>R<\/jats:italic>)\u2010 and (1\u2032<jats:italic>R<\/jats:italic>)\u2010configuration, and conversely, neither of them occurred to its (1\u2032<jats:italic>S<\/jats:italic>,2<jats:italic>S<\/jats:italic>)\u2010isomer, which caused the significantly different elimination rate in vivo. These new findings were meaningful for evaluation of the safety and efficacy of chiral drugs.<\/jats:p>","DOI":"10.1002\/jssc.202300562","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T10:53:35Z","timestamp":1698749615000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Study of the stereospecificity in the biotransformation of the four isomers of loxoprofen sodium in rats by chiral HPLC"],"prefix":"10.1002","volume":"47","author":[{"given":"Shirong","family":"Cao","sequence":"first","affiliation":[{"name":"School of Pharmacy Binzhou Medical University  Yantai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejiao","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Pharmacy Binzhou Medical University  Yantai P. R. 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