{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:21:28Z","timestamp":1780485688876,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>The level of vitamin B group in human serum is an important index of human health. Among B vitamins, cyanocobalamin in serum is unstable and its content is extremely low. Rapid and simultaneous detection of multiple B vitamins including cyanocobalamin is a challenge. Herein, we have developed a rapid and stable method that can realize the determination of thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxic acid, biotin, 5\u2010methyltetrahydrofolate, and cyanocobalamin simultaneously in 6 min. The method was established based on protein precipitation with methanol and then chromatographic separation was achieved using Waters acquity ultra\u2010high\u2010performance liquid chromatography high strength silica T3 column, which was stable and sensitive especially for cyanocobalamin. Limit of quantification, precision, trueness, and matrix effect were validated according to the European Medicines Agency and United States Food and Drug guidelines and Clinical and Laboratory Standards Institute guidelines on bioanalytical method. The limit of quantification for thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxic acid, biotin, 5\u2010methyltetrahydrofolate, and cyanocobalamin was 0.4, 0.4, 0.8, 2.0, 0.4, 0.1, 0.4, and 0.04\u00a0ng\/mL separately, respectively. Intra\u2010 and interday precisions were 1.1%\u201312.4% and 2.0%\u201313.5%, respectively. The relative errors were between 0.3% and 13.3%, and the matrix effects were between 2.6% and 10.4%.<\/jats:p>","DOI":"10.1002\/jssc.202300576","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T15:08:51Z","timestamp":1703084931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A robust method for simultaneous determination of eight B vitamins in human serum by liquid chromatography tandem mass spectrometry"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2529-428X","authenticated-orcid":false,"given":"Yu\u2010Mei","family":"Huo","sequence":"first","affiliation":[{"name":"Hangzhou Adicon Clinical Laboratories Co., Ltd  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang\u2010Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Sciences Northeastern University  Shenyang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao\u2010Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"Hangzhou Adicon Clinical Laboratories Co., Ltd  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Bo","family":"Shan","sequence":"additional","affiliation":[{"name":"Hangzhou Adicon Clinical Laboratories Co., Ltd  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Pan","sequence":"additional","affiliation":[{"name":"Hangzhou Adicon Clinical Laboratories Co., Ltd  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr11030937"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.09.049"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700598"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/1028415X.2021.2020402"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110326"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01021-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12113380"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2023.02.006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2023.117040"},{"key":"e_1_2_9_11_1","unstructured":"BellowsL MooreR. Water\u2010soluble vitamins: B\u2010complex and vitamin C (Fact Sheet No. 9.312). Food and Nutrition Series.2012."},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/46.3.225"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mog.0000203870.22706.52"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2021.110835"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/sdi.12099"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/virtualmentor.2006.8.6.cprl1-0606"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2021.01.056"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.02.033"},{"key":"e_1_2_9_19_1","article-title":"A novel HPLC method for the concurrent analysis and quantitation of seven water\u2010soluble vitamins in biological fluids (plasma and urine): a validation study and application","volume":"2012","author":"Giorgi MG","year":"2012","journal-title":"Sci World J"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2423(08)60075-X"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf060346e"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122541"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-020-03902-2"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jalm\/jfaa166"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.03.099"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123336"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9065154"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2022.107625"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202201004"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-022-04317-8"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2021.3084-3090"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122968"},{"issue":"4","key":"e_1_2_9_33_1","article-title":"Validated B vitamin quantification from lentils by selected reaction monitoring mass spectrometry","volume":"10","author":"Zhang H","year":"2020","journal-title":"BioRxiv"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.17508\/CJFST.2014.6.2.08"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3583"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2021.111490"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202300576","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:48:48Z","timestamp":1760881728000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202300576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,20]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/jssc.202300576"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202300576","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,20]]},"assertion":[{"value":"2023-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300576"}}