{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:07:42Z","timestamp":1777900062880,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["18JCYBJC95100"],"award-info":[{"award-number":["18JCYBJC95100"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>In this study, a deep eutectic solvent (DES) extraction combined with a magnetic bead ligand affinity analytical method was developed and used for \u03b1\u2010glucosidase inhibitor identification from <jats:italic>Pueraria lobata<\/jats:italic>. Several critical parameters affecting the analysis performance, including the type of DES, molar ratio, water amount, pH, salt concentration, and volume of DES, were investigated. The selected analytical sample preparation conditions were as follows. The composition of DES is choline chloride\u20101,4\u2010butanediol (1:3), the water content is 40%, pH is 7.0 and the volume of extraction solution is 2 mL. The obtained sample extraction solution was analyzed directly using \u03b1\u2010glucosidase immobilized magnetic beads (GMBs). Three \u03b1\u2010glucosidase inhibitors in <jats:italic>Pueraria lobata<\/jats:italic>, including puerarin, daidzin, and daidzein, were identified. Luteolin was used as a positive control to evaluate the method's selectivity. Results showed it could selectively bond to the GMBs in the DES. As the affinity analysis was performed directly in a DES, the solution\u2010removing process could be avoided. The intra\u2010day and inter\u2010day precisions of the method are 5.21% and 6.38%, respectively. The solvent amount was 1\/50\u20131\/2000 of that used in traditional methods.<\/jats:p>","DOI":"10.1002\/jssc.202300672","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T23:15:57Z","timestamp":1703286957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Deep eutectic solvent extraction combined with magnetic bead ligand fishing for identification of \u03b1\u2010glucosidase inhibitors from Pueraria lobata"],"prefix":"10.1002","volume":"47","author":[{"given":"Bing","family":"Li","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics Department of Natural Medicine School of Pharmacy Tianjin Medical University  Tianjin P. R. 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