{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:42:28Z","timestamp":1760920948912,"version":"build-2065373602"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T00:00:00Z","timestamp":1701561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Little or no information is available concerning online high\u2010performance liquid chromatography (HPLC) antioxidants and the antibiofilm effect of <jats:italic>Leonurus cardiaca<\/jats:italic>. Five distinct extractions of methanolic, ethyl acetate, dichloromethane, hexane, and water were obtained from <jats:italic>L. cardiaca<\/jats:italic>. In the online\u2010HPLC\u2010antioxidant analysis of all examined samples, rosmarinic acid emerged as the primary antioxidant, registering concentrations ranging from 6 to 15\u00a0ppm at wavelengths of 517 and 734\u00a0nm. Notably, the water extract exhibited robust antioxidant activity In vitro. Regarding acetylcholinesterase and butrylcholinesterase inhibition, the n\u2010hexane extract exhibited superior inhibition with values of 3.08 and 5.83 galanthamine equivalent, respectively. Except for the water extract, all tested extracts (at a concentration of 20\u00a0\u03bcg\/mL) exhibited substantial inhibitory activity against biofilm formation, in many cases superior to 80%, and reached even 94.52% against\u00a0<jats:italic>Escherichia coli<\/jats:italic>. Although less vigorous, the extracts also acted against the mature biofilm (inhibition up 76.50% against\u00a0<jats:italic>Staphylococcus aureus<\/jats:italic>). They could work against the metabolism inside an immature and mature biofilm, with inhibition percentages up to 93.18% (vs.\u00a0<jats:italic>Pseudomonas aeruginosa<\/jats:italic>) and 76.50% (vs.\u00a0<jats:italic>Acinetobacter baumannii<\/jats:italic>), respectively. Considering its significant antioxidants, enzyme inhibition, and antimicrobial activity, <jats:italic>L. cardiaca<\/jats:italic> emerges as a promising candidate for therapeutic potential.<\/jats:p>","DOI":"10.1002\/jssc.202300695","type":"journal-article","created":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T22:21:28Z","timestamp":1701642088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An analytical framework combining online high\u2010performance liquid chromatography methodologies and biological properties of different extracts of <i>Leonurus cardiaca<\/i>"],"prefix":"10.1002","volume":"47","author":[{"family":"Nilofar","sequence":"first","affiliation":[{"name":"Department of Biology Physiology and Biochemistry Laboratory Science Faculty Selcuk University  Konya Turkey"},{"name":"Department of Pharmacy Botanic Garden \u201cGiardino dei Semplici\u201d Universit\u00e0 degli Studi \u201cGabriele 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