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A simple, rapid, and economical high\u2010performance liquid chromatography\u2010ultraviolet detection (HPLC\u2010UV) method for the simultaneous determination of three ionophoric coccidiostats, namely salinomycin (SAL), maduramicin (MAD), and monensin (MON) in addition to three AAs; L\u2010tryptophan (L\u2010TRP), alpha\u2010ketoleucin (KLEU), and L\u2010valine (L\u2010VAL) in feed premixes was developed and validated. Chromatographic separation was achieved in less than 12\u00a0min using a phenyl hexyl column with a mobile phase consisting of acetonitrile\/methanol\/water (25:20:55, v\/v\/v) adjusted to pH 3 using phosphoric acid. Isocratic elution was performed at a flow rate of 1\u00a0mL\/min with UV detection at 210\u00a0nm. The method showed good linearity in the ranges 0.50\u20135.0\u00a0mg\/mL for MON, 0.20\u20132.0\u00a0mg\/mL for MAD and SAL, 10.0\u2013100.0\u00a0\u03bcg\/mL for L\u2010TRP and KLEU, and 50.0\u2013500.0\u00a0\u03bcg\/mL for VAL. The developed method was successfully applied to determine the studied analytes in feed premixes with good recoveries and precision. 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