{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:29:10Z","timestamp":1760920150859,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T00:00:00Z","timestamp":1712275200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82020108030","U21A20416"],"award-info":[{"award-number":["82020108030","U21A20416"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>Folic acid (FA) is easily photodegraded to yield 6\u2010formylpterin and pterin\u20106\u2010carboxylic acid, which can generate reactive oxygen species and result in the formation of oxidized guanine derivatives such as 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine and 8\u2010hydroxy\u2010guanosine.\u00a0In this study, we developed a simple, rapid, and sensitive liquid chromatography\u2010tandem mass spectrometry strategy for the simultaneous determination of FA photolysis products and oxidized guanine derivatives in plasma samples. Chromatographic separation was performed on a Waters HSS T3 column (2.1 \u00d7 100\u00a0mm, 5.0\u00a0\u03bcm) with gradient elution at a flow rate of 0.25\u00a0mL\/min. Plasma samples were first pretreated with 1% formic acid, followed by protein precipitation with methanol. The developed method showed good linear relationships between 1 and 2000\u00a0ng\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0&gt;\u00a00.99). The intra\u2010 and inter\u2010day precisions ranged from 2.6% to 7.5% and from 2.5% to 6.5%, respectively. Recoveries of the analytes were between 75.4% and 112.4% with the relative standard deviation\u00a0&lt;\u00a09.1%. Finally, the method was applied to quantify FA photolysis products and oxidized guanine derivatives in rats with light and non\u2010light conditions.<\/jats:p>","DOI":"10.1002\/jssc.202300763","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T03:10:35Z","timestamp":1712286635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous determination of folic acid photolysis products and oxidized guanine derivatives in plasma by liquid chromatography\u2010tandem mass spectrometry"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9600-0886","authenticated-orcid":false,"given":"Juan","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Zhengzhou University  Zhengzhou P. R. China"},{"name":"Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenchao","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengxue","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingqi","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manni","family":"He","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Zhengzhou University  Zhengzhou P. R. China"},{"name":"Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.08.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.822209"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2017.10.001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22173-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2016.08.045"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2016.01.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2015.03.038"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.80530"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2014.01.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9110735"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2cp41476g"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(02)00722-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-021-01861-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.05.005"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.10.439"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2017.11.002"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.06.014"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100417"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4244"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.121931"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac403124a"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.10.029"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2018.06.081"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.10.042"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202300763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:41:23Z","timestamp":1760870483000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202300763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jssc.202300763"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202300763","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"type":"print","value":"1615-9306"},{"type":"electronic","value":"1615-9314"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300763"}}