{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T16:49:37Z","timestamp":1781023777636,"version":"3.54.1"},"reference-count":93,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p><jats:italic>Swertia<\/jats:italic> species are common ingredients in numerous herbal remedies. It is also used to treat a wide range of illnesses and possess diverse therapeutic activities. The aim of the study is to elucidate the comprehensive metabolomics profile of <jats:italic>Swertia chirayita<\/jats:italic> and the role of various extraction methods in the phytochemical compositions of the extracts of <jats:italic>S. chirayita<\/jats:italic>, and their antioxidant and enzyme inhibitory activities. Extraction of the stems, leaves, and flowering tops of <jats:italic>S. chirayita<\/jats:italic> was performed by maceration, infusion, and soxhlation using methanol and water as solvent. Extracts were subjected to phytochemical profiling by a liquid\u2010chromatographic system. Antioxidant and enzyme inhibitory activity was carried out. The metabolomics profiling showed that a diverse range of specialized metabolites were present in the stems and leaves &amp; flowering tops of the plant. All the extracts showed substantial antioxidant and enzyme inhibitory activities further confirmed by molecular docking studies. This study appraised the use of <jats:italic>S. chirayita<\/jats:italic> aerial parts as a potential antioxidant and its therapeutic application in various chronic illnesses including Alzheimer's disease, diabetes, and other skin\u2010related disorders.<\/jats:p>","DOI":"10.1002\/jssc.202300795","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:00:55Z","timestamp":1705017655000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Metabolomics\u2010based pharmaceutical evaluation of different parts of <i>Swertia chirayita<\/i> (Roxb.) Buch.\u2010Ham. ex C.B. Clarke from the western Himalayas"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5125-3791","authenticated-orcid":false,"given":"Devesh","family":"Tewari","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Sciences Delhi Pharmaceutical Sciences and Research University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sweta","family":"Bawari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Amity Institute of Pharmacy Amity University  Noida India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh T","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Career Services Edvancer \u2010 a part of Global University System, India  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawan","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Shri Vile Parle Kelavani Mandal's Institute of Pharmacy  Dhule India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aryalaxmi","family":"M","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry School of Pharmaceutical Sciences Delhi Pharmaceutical Sciences and Research University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Czi\u00e1ky","sequence":"additional","affiliation":[{"name":"Agricultural and Molecular Research and Service Institute University of Ny\u00edregyh\u00e1za  Nyiregyhaza Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"Jeko","sequence":"additional","affiliation":[{"name":"Agricultural and Molecular Research and Service Institute University of Ny\u00edregyh\u00e1za  Nyiregyhaza Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Lazarova","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Pharmacy Medical University\u2010Sofia  Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"G\u00f6khan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science Sel\u00e7uk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"308","article-title":"A review of swertia chirayita (Gentianaceae) as a traditional medicinal plant","volume":"6","author":"Kumar V","year":"2015","journal-title":"Front Pharmacol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-1984.2006.00150.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","unstructured":"PantN JainDC BhakuniRS.Phytochemicals from genus Swertia and their biological activities.2000.","DOI":"10.1002\/chin.200103247"},{"key":"e_1_2_9_5_1","first-page":"199","article-title":"Therapeutic Swertia chirayita\u2013an overview","volume":"5","author":"Patil K","year":"2013","journal-title":"Res J Pharmacogn Phytochem"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tfp.2022.100203"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13659-020-00277-7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2012.729037"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1994-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2013.1373"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jams.2012.10.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298-014-0276-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2015.1004175"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116157"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/22311866.2015.1044703"},{"key":"e_1_2_9_16_1","first-page":"1439","article-title":"Phenolic profiling, antioxidants, multivariate, and enzyme inhibitory properties of wild Himalayan fig (Ficus palmata Forssk.): a potential candidate for designing innovative nutraceuticals and related products","author":"Tewari D","year":"2020","journal-title":"Anal Lett"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.08.020"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00290"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6FO01847E"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_9_21_1","first-page":"57","article-title":"Python: a programming language for software integration and development","volume":"17","author":"Sanner MF","year":"1999","journal-title":"J Mol Graph Model"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2018.03.001"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2022.05.018"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2022.134119"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570180814666170915162232"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.11113\/mjfas.v17n2.2059"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26103043"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.10.069"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11041089"},{"key":"e_1_2_9_31_1","article-title":"In Silico and in vitro study of the ethanol extract of the White Garland Lily (Hedychium coronarium J. Koenig) as a tyrosinase inhibitor","volume":"7","author":"Suharti N","year":"2023","journal-title":"Trop J Nat Prod Res"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2018.05.028"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-022-00841-x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2019.108610"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6792069"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr8101222"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117004"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2020.1754849"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.012330"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.36722"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-023-09790-0"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgr.2021.08.003"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107986"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-023-01718-6"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154730"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2015.03.002"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110561"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.08.001"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.01.053"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/b009171p"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27175590"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2017.07.024"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.04.035"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11696-018-0415-9"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8090343"},{"key":"e_1_2_9_56_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2018\/7612172","article-title":"Mechanisms and disease pathogenesis underlying metal\u2010induced oxidative stress","volume":"2018","author":"Chen P","year":"2018","journal-title":"Oxid Med Cell Longev"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867053764635"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr10010132"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1080\/23312025.2019.1710431"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejbt.2015.08.007"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.619373"},{"key":"e_1_2_9_62_1","article-title":"Current state of knowledge on the antioxidant effects and mechanisms of action of polyphenolic compounds","volume":"16","author":"Lv Q","year":"2021","journal-title":"Nat Prod Commun"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26175377"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13394"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2014.03.026"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.11.046"},{"key":"e_1_2_9_67_1","first-page":"1","article-title":"Multidirectional insights into the phytochemical, biological, and multivariate analysis of extracts from the aerial part of Swertia perennis Linnaeus","author":"He X","year":"2022","journal-title":"Nat Prod Res"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.04.058"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-8490.157997"},{"key":"e_1_2_9_70_1","first-page":"1975","article-title":"Comparative phytochemical, hepatoprotective and antioxidant activities of various samples of Swertia Chirayita collected from various cities of Pakistan","volume":"27","author":"Mahmood S","year":"2014","journal-title":"Pak J Pharm Sci"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-78782-5"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.1c00538"},{"key":"e_1_2_9_73_1","first-page":"3092","article-title":"Acetylcholinesterase and butyrylcholinesterase anchoring in murine ocular compartments, role of Colq and PRiMA","volume":"59","author":"Abitbol MM","year":"2018","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2012.12.054"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/717232"},{"key":"e_1_2_9_76_1","first-page":"25","article-title":"Cholinesterase inhibitory, anti\u2010oxidant and anti\u2010tyrosinase activities of three Iranian species of Dracocephalum","volume":"6","author":"Khodaei M","year":"2019","journal-title":"Res J Pharmacogn"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201401595"},{"key":"e_1_2_9_78_1","first-page":"1","article-title":"Tyrosinase inhibitory effect and antioxidative activities of fermented and ethanol extracts of Rhodiola rosea and Lonicera japonica","volume":"2013","author":"Chen Y\u2010S","year":"2013","journal-title":"Sci World J"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2015.03.012"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.849585"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2012.07.005"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.08.004"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2014.973964"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1002\/hsr2.389"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41514-020-00049-0"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.06.009"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2694"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.04.017"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.3390\/app10030947"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2013.00038"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42717"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03190598"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.07.042"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.112997"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.202300795","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:49:16Z","timestamp":1760881756000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.202300795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":93,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/jssc.202300795"],"URL":"https:\/\/doi.org\/10.1002\/jssc.202300795","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300795"}}