{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T17:31:17Z","timestamp":1776274277659,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073954"],"award-info":[{"award-number":["82073954"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2021MH300"],"award-info":[{"award-number":["ZR2021MH300"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>An effective method by high\u2010speed countercurrent chromatography coordinated with silver nitrate for the preparative separation of sterones and triterpenoid saponins from <jats:italic>Achyranthes bidentata<\/jats:italic> Blume was developed. Methyl tert\u2010butyl ether\/n\u2010butanol\/acetonitrile\/water (4:2:3:8, v\/v\/v\/v) was selected for 20\u2010hydroxyecdysone (compound <jats:bold>1<\/jats:bold>), chikusetsusaponin IVa methyl ester (compound <jats:bold>4<\/jats:bold>), 2\u2032\u2010glycan\u201011\u2010keto\u2010pigmented saponin V (compound <jats:bold>5<\/jats:bold>), as well as a pair of isomers of 25<jats:italic>S<\/jats:italic>\u2010inokosterone (compound <jats:bold>2<\/jats:bold>) and 25<jats:italic>R<\/jats:italic>\u2010inokosterone (compound <jats:bold>3<\/jats:bold>), which were further purified by silver nitrate coordinated high\u2010speed countercurrent chromatography. What is more, dichloromethane\/methanol\/isopropanol\/water (6:6:1:4, v\/v\/v\/v) was applied for calenduloside E (compound <jats:bold>6<\/jats:bold>), 3<jats:italic>\u03b2<\/jats:italic>\u2010[(O\u2010<jats:italic>\u03b2<\/jats:italic>\u2010<jats:sc>d<\/jats:sc>\u2010glucuronopyranosyl)\u2010oxy]\u2010oleana\u201011,13\u2010dien\u201028\u2010oic acid (compound <jats:bold>7<\/jats:bold>), zingibroside R1 (compound <jats:bold>8<\/jats:bold>) and chikusetsusaponin IVa (compound <jats:bold>9<\/jats:bold>). Adding Ag<jats:sup>+<\/jats:sup> to the solvent system resulted in unique selectivity for 25<jats:italic>R<\/jats:italic>\/25<jats:italic>S<\/jats:italic> isomers of inokosterone, which increased the complexing capability and stability of Ag<jats:sup>+<\/jats:sup> coordinated 25<jats:italic>S<\/jats:italic>\u2010inokosterone, as well as the <jats:italic>\u03b1<\/jats:italic> value between them. These results were further confirmed by the computational calculation of geometry optimization and frontier molecular orbitals assay. Comprehensive mass spectrometry and nuclear magnetic resonance analysis demonstrated the structures of the obtained compounds.<\/jats:p>","DOI":"10.1002\/jssc.202300901","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:30:25Z","timestamp":1712881825000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Purification of triterpenoid saponins and 25<i>R<\/i>\/25<i>S<\/i>\u2010inokosterone from <i>Achyranthes bidentata<\/i> Bl. by high\u2010speed countercurrent chromatography coupled silver nitrate coordination"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8376-7301","authenticated-orcid":false,"given":"Tong","family":"Niu","sequence":"first","affiliation":[{"name":"Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences) Jinan P. 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