{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:34:17Z","timestamp":1760920457209,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800289"],"award-info":[{"award-number":["31800289"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2024JC\u2010YBMS\u2010787"],"award-info":[{"award-number":["2024JC\u2010YBMS\u2010787"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>In this study, we employed a combination approach for the preparative separation of constituents from <jats:italic>Ginkgo biloba<\/jats:italic> L. leaves. It involved multi\u2010stage solvent extractions utilizing two\u2010phase multi\u2010solvent systems and countercurrent chromatography (CCC) separations using three different solvent systems. The <jats:italic>n<\/jats:italic>\u2010heptane\/ethyl acetate\/water (1:1:2, v\/v) and <jats:italic>n<\/jats:italic>\u2010heptane\/ethyl acetate\/methanol\/water (HepEMWat, 7:3:7:3, v\/v) solvent systems were screened out as extraction systems. The polarities of the upper and lower phases in the multi\u2010solvent systems were adjustable, enabling the effectively segmented separation of complex constituents in <jats:italic>G. biloba<\/jats:italic> L. The segmented products were subsequently directly utilized as samples and separated using CCC with the solvent systems acetate\/<jats:italic>n<\/jats:italic>\u2010butanol\/water (4:1:5, v\/v), HepEMWat (5:5:5:5, v\/v), and HepEMWat (9:1:9:1, v\/v), respectively. As a result, a total of 11 compounds were successfully isolated and identified from a 2\u00a0g methanol extract of <jats:italic>G. biloba<\/jats:italic> L through two\u2010stage extraction and three CCC separation processes; among them, nine compounds exhibited high\u2010performance liquid chromatography purity exceeding 85%.<\/jats:p>","DOI":"10.1002\/jssc.202400234","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T02:54:54Z","timestamp":1721012094000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Preparative separation of the constituents from <i>Ginkgo biloba<\/i> L. leaves by a combination of multi\u2010stage solvent extraction and countercurrent chromatography"],"prefix":"10.1002","volume":"47","author":[{"given":"Zhuo","family":"Liu","sequence":"first","affiliation":[{"name":"College of Chemistry &amp; Pharmacy Northwest A&amp;F University  Yangling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Chemistry &amp; 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