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BE\u2010PEO surface\u2010modified silica was hydrolyzed and subsequent <jats:sup>1<\/jats:sup>H nuclear magnetic resonance analysis of hydrolysis products confirmed the successful formation of BE\u2010PEO bonded surface. Silica particles exhibiting 3\u00a0\u00b5m diameters and 1000 \u00c5 nominal pore diameters were selected as a base material for this work out of the critical need to improve analytical capabilities for the testing of cell and gene therapy drug products. Accelerated high pH aging study revealed significant enhancement in column stability. Multi\u2010angle light scattering noise measurements showed inordinately lower baseline noise. Moreover, we evaluated the chromatographic performance of BE\u2010PEO silica\u2010packed columns through separations of a protein test mixture, DNA ladder, monoclonal antibody\u2010based therapeutics, and adeno\u2010associated viruses. BE\u2010PEO silica columns demonstrated high resolution, high recovery separations that were confirmed to be reproducible and capable of extended column lifetimes and exhibited low ionic and hydrophobic secondary interactions. In summary, BE\u2010PEO silica particles have yielded a new level of performance, improved base stability, and inherently lower baseline noise. These novel widepore particles will facilitate more sensitive size\u2010based detection and characterization of large biologics in the form of advanced gene therapy products.<\/jats:p>","DOI":"10.1002\/jssc.202400541","type":"journal-article","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T02:48:37Z","timestamp":1729306117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Bridged Ethylene Polyethylene Oxide Surfaces to Improve Packing Materials for Widepore Size Exclusion Chromatography"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9375-3508","authenticated-orcid":false,"given":"Kristine Joy","family":"Camacho","sequence":"first","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oksana","family":"Tchoul","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehong","family":"Xu","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Finny","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lavelay","family":"Kizekai","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"McLaughlin","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Byrd","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balasubrahmanyam","family":"Addepalli","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MingCheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Lauber","sequence":"additional","affiliation":[{"name":"Consumables and Lab Automation Waters Corporation Milford Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,18]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"K. 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