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However, it is time\u2010consuming and vulnerable to interference to conduct the traditional metabolism approach by applying organisms or biomaterials. Herein, a bionic technology was established by combining online electrochemistry\u2010mass spectrometry (EC\u2010MS) with offline electrochemistry\u2010liquid chromatography\u2010mass spectrometry (EC\u2010LC\u2010MS) to investigate the oxidative transformation and metabolic processes of the active phenolic acids (including salvianolic acid A, caffeic acid, 3, 5\u2010<jats:italic>O<\/jats:italic>\u2010dicaffeoylquinic acid, ferulic acid, salvianic acid A, and protocatechuic acid). Phase I metabolism of the phenolic acids were simulated by applying a three\u2010electrode controlled potential electrochemical reactor with a boron\u2010doped diamond electrode, with glutathione mixed into the oxidative products simultaneously for obtaining the phase II metabolites. Finally, structural characterization of the simulated metabolites of the phenolic acids was achieved successfully, including hydroxylation, methylation, demethylation, decarboxylation, etc. It was revealed that the simulated metabolism process based on an electrochemical system was effective in yielding a wide variety of metabolites for these compounds, which was also compared with the metabolism results applying rat liver microsomes. Consequently, this bionic technology is expected to be a powerful tool to investigate the material basis for the efficacy of active ingredients of natural products.<\/jats:p>","DOI":"10.1002\/jssc.70006","type":"journal-article","created":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T01:55:17Z","timestamp":1731117317000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Advanced Bionic Technology Combining Online Electrochemistry\u2010Mass Spectrometry and Offline Electrochemistry\u2010Liquid Chromatography\u2010Mass Spectrometry for Simulating and Characterizing Metabolic Processes of Bioactive phenolic acids in Natural Products"],"prefix":"10.1002","volume":"47","author":[{"given":"Kunze","family":"Du","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis Tianjin University of Traditional Chinese 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