{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:44:30Z","timestamp":1792014270207,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T00:00:00Z","timestamp":1730851200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["T2192971"],"award-info":[{"award-number":["T2192971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Thrombin was immobilized on magnetic core\u2010shell mesoporous silica nanoparticles (Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>@nSiO<jats:sub>2<\/jats:sub>@mSiO<jats:sub>2<\/jats:sub>) for the first time and used to screen enzyme inhibitors from extracts of <jats:italic>Notopterygium incisum<\/jats:italic>. The immobilized thrombin was characterized by Fourier\u2010transform infrared spectroscopy, transmission electronic microscopy, thermal gravimetric analysis, and vibrating sample magnetometry. The results demonstrated that the immobilized thrombin possessed good thermal stability and high\u2010temperature resistance for feasible storage and reuse. Two ligands, notopterol (<jats:bold>1<\/jats:bold>) and isoimperatorin (<jats:bold>2<\/jats:bold>) were fished out from the extract of <jats:italic>N. incisum<\/jats:italic> using the immobilized thrombin. They inhibited thrombin with IC<jats:sub>50<\/jats:sub> values of 59.35\u00a0\u00b1\u00a01.420 and 107.8\u00a0\u00b1\u00a01.660\u00a0\u00b5M, respectively. This is the first report of thrombin\u2010inhibitory activity associated with notopterol and isoimperatorin. Meanwhile, the inhibition mechanism of both compounds was investigated by enzyme kinetic analysis and molecular docking. The method used enables rapid screening for thrombin inhibitors from medicinal plants, providing an efficient tool for the discovery of anti\u2010thrombotic active compounds.<\/jats:p>","DOI":"10.1002\/jssc.70021","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T08:30:15Z","timestamp":1730881815000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Thrombin Immobilized on Magnetic Core\u2010Shell Mesoporous Silica Nanoparticles for Screening of the Enzyme Inhibitors From <i>Notopterygium Incisum<\/i>"],"prefix":"10.1002","volume":"47","author":[{"given":"Qiang","family":"Chang","sequence":"first","affiliation":[{"name":"Chengdu Institute of Biology Chinese Academy of Sciences  Chengdu China"},{"name":"Department of Chemistry and Biochemistry University of Chinese Academy of Sciences  Beijing 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