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This work proposes, for the first time, an analytical method based on a switchable hydrophilicity solvent as an extraction phase for the determination of cortisol and testosterone in oral fluid (OF) by high\u2010performance liquid chromatography with diode\u2010array detection. The optimized extraction conditions consisted of 1000\u00a0\u00b5L of OF, 100\u00a0\u00b5L of decanoic acid solution (65\u00a0mg\/mL), 170\u00a0\u00b5L of Na<jats:sub>2<\/jats:sub>CO<jats:sub>3<\/jats:sub>, 900\u00a0\u00b5L of H<jats:sub>2<\/jats:sub>SO<jats:sub>4<\/jats:sub> and 150\u00a0\u00b5L of acetonitrile for dilution. The method was validated, and coefficients of determination higher than 0.9926, the limit of detection of 4.55\u00a0ng\/mL and the limit of quantification of 15.00\u00a0ng\/mL were obtained. Intra\u2010day precision varied from 5.6% to 11.9%, inter\u2010day precision ranged from 6.1% to 13.5%, and relative recoveries ranged from 98.9% to 104.6% for cortisol, and 89.1% to 103.9% for testosterone. This methodology was successfully applied to five OF samples from volunteers. Moreover, the greenness of this methodology was evaluated based on the sample preparation metric of sustainability achieving a global score of 7.37 which can be considered sustainable.<\/jats:p>","DOI":"10.1002\/jssc.70022","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T04:30:45Z","timestamp":1731040245000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effervescent\u2010assisted Liquid\u2010phase Microextraction Employing Switchable Hydrophilicity Solvent for the Determination of Cortisol and Testosterone in Oral Fluid by High\u2010Performance Liquid Chromatography\u2010Diode Array Detection"],"prefix":"10.1002","volume":"47","author":[{"given":"Carolina Silveira","family":"Dalanhol","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioci\u00eancias Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre  Porto Alegre 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