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By utilizing the ultra\u2010affinity between Colicin L7 DNase (CL7) and its homologous immune protein 7 (Im7), CL7\u2010tagged \u03b1<jats:sub>1A<\/jats:sub>\u2010AR was oriented immobilized to the Im7\u2010coated silica gel surface. With the \u03b1<jats:sub>1A<\/jats:sub>\u2010AR immobilized column in hand, the active compounds in GFF targeted to \u03b1<jats:sub>1A<\/jats:sub>\u2010AR were screened, and the binding procedures were analyzed. The composite characterization demonstrated that the \u03b1<jats:sub>1A<\/jats:sub>\u2010AR can be immobilized to the chromatographic stationary phase with good specificity and stability in 3 weeks. Paeoniflorin, cinnamic acid, and paeonol were identified as the active compounds in GFF targeted to \u03b1<jats:sub>1A<\/jats:sub>\u2010AR. Among them, cinnamic acid and paeonol have the same binding site on \u03b1<jats:sub>1A<\/jats:sub>\u2010AR as the specific drug tamsulosin. The binding parameters obtained by frontal analysis and injection amount\u2010dependent analysis were consistent in the same concentration range. Collectively, these results indicated that the \u03b1<jats:sub>1A<\/jats:sub>\u2010AR chromatographic column synthesized by a novel immobilized method was capable of screening and analyzing the functional compounds from the complex matrix, which provided an alternative for rapid screening and analysis to traditional ethnic drugs.<\/jats:p>","DOI":"10.1002\/jssc.70053","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T23:42:25Z","timestamp":1733874145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Discovering the Bioactive Compounds Binding to \u03b1<sub>1A<\/sub>\u2010Adrenergic Receptor in Guizhi Fuling Formula and Revealing Their Interactions by Immobilizing the Receptor Through Colicin L7 DNase\/Immunity Protein 7 Ultra\u2010Affinity System"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6459-1767","authenticated-orcid":false,"given":"Xu","family":"Ji","sequence":"first","affiliation":[{"name":"Engineering Research Center of Tibetan Medicine Detection Technology Ministry of Education, Xizang Minzu University  Xianyang P. 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