{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:52:20Z","timestamp":1786636340096,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201023\u20100508"],"award-info":[{"award-number":["APVV\u201023\u20100508"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201022\u20100313"],"award-info":[{"award-number":["APVV\u201022\u20100313"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Recently, proteinogenic amino acids have become very interesting molecules, accompanied by a large variety of metabolic processes in humans and are associated with various diseases. In the era of system biology, including a broad spectrum of associated disciplines (e.g., metabolomics, lipidomics, proteomics, etc.), the possibility of identifying trustworthy biomarkers of diseases becomes much more likely. Changes in amino acid levels in plasma, serum, or cerebrospinal fluid reflect physiological or pathological conditions and, therefore, their regular monitoring can lead to early detection of the occurrence of a disease. Therefore, the exact determination of amino acids in biological fluids is of great importance. However, it is necessary to dispose with an effective, accurate, precise, selective, and robust analytical method. This protocol describes the complex procedure of amino acid analysis based on a combination of UHPLC with single quadrupole MS. The protocol presents a highly reproducible and robust methodology that has already been established in the quality control of biopharmaceuticals and determination of proteinogenic amino acids in urine in our laboratory. Here, the application potential is extended to the most frequently investigated biological fluid, that is, plasma and to the cerebrospinal fluid, which is investigated in many neurological conditions.<\/jats:p>","DOI":"10.1002\/jssc.70089","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T23:39:46Z","timestamp":1738798786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Protocol for Determination of Proteinogenic Amino Acids in Biological Fluids by the High\u2010Speed UHPLC\u2010MS Method: Application on Transgenic Spontaneously Hypertensive Rat\u201024 Plasma and Cerebrospinal Fluid Samples"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4736-4592","authenticated-orcid":false,"given":"Juraj","family":"Piestansky","sequence":"first","affiliation":[{"name":"Institute of Neuroimmunology Slovak Academy of Sciences  Bratislava Slovak Republic"},{"name":"Department of Galenic Pharmacy Faculty of Pharmacy Comenius University in Bratislava  Bratislava Slovak Republic"},{"name":"Toxicological and Antidoping Center Faculty of Pharmacy Comenius University in Bratislava  Bratislava Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika","family":"Olesova","sequence":"additional","affiliation":[{"name":"Institute of Neuroimmunology Slovak Academy of Sciences  Bratislava Slovak Republic"},{"name":"Institute of Experimental Endocrinology Biomedical Research Center  Bratislava Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3223-8705","authenticated-orcid":false,"given":"Petra","family":"Majerova","sequence":"additional","affiliation":[{"name":"Institute of Neuroimmunology Slovak Academy of Sciences  Bratislava Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Chalova","sequence":"additional","affiliation":[{"name":"Institute of Neuroimmunology Slovak Academy of Sciences  Bratislava Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrej","family":"Kovac","sequence":"additional","affiliation":[{"name":"Institute of Neuroimmunology Slovak Academy of Sciences  Bratislava Slovak Republic"},{"name":"Department of Galenic Pharmacy Faculty of Pharmacy Comenius University in Bratislava  Bratislava Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-009-0269-0"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21548"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2013.06.015"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-010-0609-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.02.019"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.05.001"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2021.147704"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32205-4"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-7568(21)00145-8"},{"key":"e_1_2_11_11_1","unstructured":"M.Prince A.Comas\u2010Herrera M.Knapp M.Guerchet andMKaragiannidou \u201cWorld Alzheimer Report 2016: Improving Healthcare for People Living With Dementia. Coverage Quality and Costs Now and in the Future \u201d Alzheimer's Disease International 2016 https:\/\/www.alzint.org\/u\/WorldAlzheimerReport2016.pdf."},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/alzrt198"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13901-z"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.11.021"},{"key":"e_1_2_11_15_1","first-page":"294","article-title":"Metabolomics for the Masses: The Future of Metabolomics in a Personalized World","volume":"3","author":"Trivedi D. K.","year":"2018","journal-title":"New Horizons in Translational Medicine"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2018.08.016"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.05.011"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-006-0409-8"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024143"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0254-6272(15)30063-7"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.05.053"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nutd.2016.55"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2021.07.003"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-019-0581-z"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24183345"},{"key":"e_1_2_11_26_1","unstructured":"\u201cAnimal Research: Reporting ofIn VivoExperiments \u201dhttps:\/\/nc3rs.org.uk\/arrive\u2010guidelines."},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11051461"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1055\/b-005-148942"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181c47cc2"},{"key":"e_1_2_11_30_1","unstructured":"A.Kassambara \u201crstatix: Pipe\u2010Friendly Framework for Basic Statistical Tests. R package version 0.7.2. \u201d 2023 https:\/\/rpkgs.datanovia.com\/rstatix\/."},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.02472"},{"key":"e_1_2_11_32_1","unstructured":"U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) and Center for Veterinary Medicine (CVM) Bioanalytical Method Validation Guidance for Industry Guidance Document (Center for Drug Evaluation and Research Center for Veterinary Medicine 2018)."},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2021.03.004"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12030272"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.200"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b04623"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2AY01588A"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.36922\/gpd.3294"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.10.015"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2023.1219718"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241411236"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13741"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5472792"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2023.1214932"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2019-16631"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70089","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70089","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70089","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:56:14Z","timestamp":1786632974000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70089"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/jssc.70089"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70089","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70089"}}