{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T10:26:11Z","timestamp":1763202371320,"version":"3.45.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vitamin D plays a crucial role in skeletal metabolism and is implicated in various diseases. Several studies have indicated that human vitamin D status can be evaluated through urine hydroxyvitamin D levels, in addition to plasma hydroxyvitamin D concentrations. In this study, we developed a novel method for the detection of trace amounts of 25\u2010hydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    [25(OH)D\n                    <jats:sub>3<\/jats:sub>\n                    ] and 24,25\u2010dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    [24,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    ] in urine. This method is based on high\u2010performance liquid chromatography coupled with atmospheric pressure chemical ion source\u2010tandem mass spectrometry, integrated with packed\u2010fibers solid\u2010phase extraction. The composite polystyrene\/graphene oxide (PS\/GO) nanofibers were synthesized as the sorbent for concentrating 25(OH)D\n                    <jats:sub>3<\/jats:sub>\n                    and 24,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    , achieving a twentyfold increase in sensitivity when analyzing human urine samples. Fourier\u2010transform infrared spectroscopy, X\u2010ray diffraction, and energy\u2010dispersive X\u2010ray spectroscopy mapping analysis were employed to characterize GO, PS, and PS\/GO. The results indicated that the composite nanofibers were successfully synthesized. In addition, we investigated the relationship between the extraction performance of the fibers and the electrospinning process at varying flow rates of spinning solution through morphological studies and Brunauer\u2010Emmett\u2010Teller surface area analysis. During the extraction process, purification, concentration, and desorption were accomplished within a single step. The established method demonstrated excellent sensitivity and efficiency. Under optimal conditions, limits of detection (signal\u2010to\u2010noise ratio = 3) were found to be 0.33\u00a0ng\/mL for 25(OH)D\n                    <jats:sub>3<\/jats:sub>\n                    and 0.19\u00a0ng\/mL for 24,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    ; furthermore, linearity was deemed acceptable across urine samples. Recovery rates ranged from 89.5% to 109.7% for 25(OH)D\n                    <jats:sub>3<\/jats:sub>\n                    and from 90.3% to 103.1% for 24,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    , respectively.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70130","type":"journal-article","created":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T06:21:56Z","timestamp":1744438916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Determination of 25\u2010Hydroxyvitamin D\n                    <sub>3<\/sub>\n                    and 24,25\u2010Dihydroxyvitamin D\n                    <sub>3<\/sub>\n                    in Human Urine Based on Polystyrene\/Graphene Oxide Packed\u2010Fibers Solid\u2010Phase Extraction Coupled With High\u2010Performance Liquid Chromatography and Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"48","author":[{"given":"Qing","family":"Han","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Engineering Jiangsu Food and Pharmaceutical Science College  Huaian China"},{"name":"School of Biological Sciences &amp; Medical Engineering Southeast University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Engineering Jiangsu Food and Pharmaceutical Science College  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuejun","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Biological Sciences &amp; Medical Engineering Southeast University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guozhe","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Biological Sciences &amp; Medical Engineering Southeast University  Nanjing China"},{"name":"College of Food Jiangsu Food and Pharmaceutical Science College  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jiangsu Food and Pharmaceutical Science College  Huaian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Geng","sequence":"additional","affiliation":[{"name":"Food and Pharmaceutical Research Institute Jiangsu Food and Pharmaceutical Science College  Huaian 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