{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T23:01:04Z","timestamp":1784329264958,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Anthraquinones are the main constituents of seeds of\n                    <jats:italic>Cassia obtusifolia<\/jats:italic>\n                    L., which have been used extensively in traditional medicine around the world to cure a variety of illnesses. This work used linear gradient counter\u2010current chromatography to purify anthraquinones successfully. Two gradient mobile phases were employed: the lower phases of the solvent system combination of\n                    <jats:italic>n\u2010<\/jats:italic>\n                    hexane\/ethyl acetate\/methanol\/water (\n                    <jats:italic>n<\/jats:italic>\n                    \u2010Hex\/EtOAc\/MeOH\/H\n                    <jats:sub>2<\/jats:sub>\n                    O) (5:5:5:5, v\/v) and (5:5:8:2, v\/v). The solvent systems employed for the inner\u2010recycling separation were\n                    <jats:italic>n<\/jats:italic>\n                    \u2010Hex\/EtOAc\/MeOH\/H\n                    <jats:sub>2<\/jats:sub>\n                    O (5:5:5.5:4.5, v\/v) and (5:5:7:3, v\/v). Additionally,\n                    <jats:italic>n\u2010<\/jats:italic>\n                    hexane\/ethanol\/water (1:1:0.2, v\/v) was utilized as the solvent system to separate the fraction using the offline two\u2010dimensional separation mode. Lastly, high\u2010performance liquid chromatography revealed that nine anthraquinones, including aurantio\u2010obtusin (\n                    <jats:bold>1<\/jats:bold>\n                    ), chryso\u2010obtusin (\n                    <jats:bold>2<\/jats:bold>\n                    ), obtusin (\n                    <jats:bold>3<\/jats:bold>\n                    ), btusifolin (\n                    <jats:bold>4<\/jats:bold>\n                    ), emodin (\n                    <jats:bold>5<\/jats:bold>\n                    ), rubrofusarin (\n                    <jats:bold>6<\/jats:bold>\n                    ), toralactone (\n                    <jats:bold>7<\/jats:bold>\n                    ), chrysophanol (\n                    <jats:bold>8<\/jats:bold>\n                    ) and physcion (\n                    <jats:bold>9<\/jats:bold>\n                    ) were effectively obtained with purities over 98%. The established technique was used to isolate a series of wide\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    \u2010value anthraquinones from seeds of\n                    <jats:italic>C. obtusifolia<\/jats:italic>\n                    L. The developed approach demonstrated significant potential for the isolation of complex components with broad polarities from other natural products.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70131","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:23:06Z","timestamp":1744244586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An Efficient Isolation and Purification of Broad Partition Coefficient Range of Anthraquinones From Seeds of\n                    <i>Cassia obtusifolia<\/i>\n                    L. by Linear Gradient Counter\u2010Current Chromatography Coupled With Inner\u2010Recycling Mode"],"prefix":"10.1002","volume":"48","author":[{"given":"Zhengfu","family":"Gong","sequence":"first","affiliation":[{"name":"College of Pharmacy Food Resources Development and Health Product Creation International Joint Laboratory\/Biological Engineering Technology Innovation Center of Shandong Province Heze Branch of Qilu University of Technology (Shandong Academy of Sciences)  Heze China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Huang","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shandong University  Jinan China"},{"name":"Advanced Medical Research Institute Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinping","family":"Li","sequence":"additional","affiliation":[{"name":"Area Studies Center College of Foreign Languages Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammara","family":"Sohail","sequence":"additional","affiliation":[{"name":"College of Pharmacy Food Resources Development and Health Product Creation International Joint Laboratory\/Biological Engineering Technology Innovation Center of Shandong Province Heze Branch of Qilu University of Technology (Shandong Academy of Sciences)  Heze China"},{"name":"Institute of Chemistry University of Okara  Okara Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesham R.","family":"El\u2010Seedi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Islamic University of Madinah  Madinah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeonghill","family":"Park","sequence":"additional","affiliation":[{"name":"College of Pharmacy Food Resources Development and Health Product Creation International Joint Laboratory\/Biological Engineering Technology Innovation Center of Shandong Province Heze Branch of Qilu University of Technology (Shandong Academy of Sciences)  Heze China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Sun","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shandong University  Jinan China"},{"name":"Advanced Medical Research Institute Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2156-4548","authenticated-orcid":false,"given":"Daijie","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Food Resources Development and Health Product Creation International Joint Laboratory\/Biological Engineering Technology Innovation Center of Shandong Province Heze Branch of Qilu University of Technology (Shandong Academy of Sciences)  Heze 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