{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:25:48Z","timestamp":1772252748947,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2503803"],"award-info":[{"award-number":["2022YFC2503803"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Monotherapy or combination therapy with anti\u2010seizure medications (ASMs) remains the cornerstone of epilepsy treatment. Therapeutic drug monitoring is important for individualized ASM treatment and for optimizing outcomes in patients with epilepsy. Therefore, the simultaneous and accurate quantification of ASMs is necessary and helpful for clinical use. In this study, a new method was developed to simultaneously quantify carbamazepine, lacosamide, lamotrigine, levetiracetam, oxcarbazepine, perampanel, sodium valproate, topiramate, and an active metabolite of oxcarbazepine, 10, 11\u2010dihydro\u201010\u2010hydroxycarbamazepine, in human plasma by high\u2010performance liquid chromatography\u2010tandem mass spectrometry using the positive\/negative ion\u2010switching ionization mode. The method was assessed for accuracy, precision, linearity, recovery, and matrix effects according to the criteria of the U.S. Food and Drug Administration Bioanalytical Method Validation Guidelines for Industry for Bioanalytical Methods. The lower limit of quantification was determined to be 0.001\u201325\u00a0\u00b5g\/mL for each analyte. The intra\u2010 and inter\u2010day precision values had &lt;14.6% coefficients of variation, and the accuracy ranged from 85.3% to 113% at the three levels of quality control. In addition, this method was successfully applied to therapeutic drug monitoring of ASMs in patients with epilepsy. This method allows for simple preparation, accurate quantification, and the determination of different ASMs without requiring analytical method switching, making it particularly suitable for therapeutic drug monitoring in busy clinical and hospital laboratory settings.<\/jats:p>","DOI":"10.1002\/jssc.70151","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:25:44Z","timestamp":1745367944000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous Determination of Multiple Anti\u2010Seizure Medications in Human Plasma Using High\u2010Performance Liquid Chromatography\u2010Tandem Mass Spectrometry with Positive\/Negative Ion\u2010Switching Ionization Mode for Therapeutic Drug Monitoring"],"prefix":"10.1002","volume":"48","author":[{"given":"Yu\u2010Fei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Huashan Hospital Fudan University  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