{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T02:20:21Z","timestamp":1788315621570,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Risdiplam is a new therapeutic agent developed to treat spinal muscular atrophy (SMA), a genetic neurodegenerative disease caused by mutations in the\n                    <jats:italic>SMN1<\/jats:italic>\n                    gene. Unlike previous invasive therapies, risdiplam offers the advantage of oral administration, significantly improving patient comfort and accessibility. The review provides information on an SMA historical overview, breakthrough therapies of the development, and design of the methods used to treat SMA. We then focus on its structure and physicochemical properties. The analysis of risdiplam concentrates on developing improved analytical methods for the precise quantification of risdiplam and its metabolites by high\u2010performance liquid chromatography with mass spectrometry in biological samples using octadecyl stationary phases. For sample preparation, only the protein precipitation method was used. Challenges associated with the risdiplam analytics include developing a highly sensitive and selective method in biological matrices and dealing with potential interferences from the biological matrix. Future research should focus on improving analytical methods, investigating metabolite activity, and expanding our knowledge of its long\u2010term effects.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70173","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T21:59:07Z","timestamp":1747087147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Short Review on Currently Used Sample Preparation and Determination Methods of Risdiplam"],"prefix":"10.1002","volume":"48","author":[{"given":"Natalia","family":"Bali\u0144ska","sequence":"first","affiliation":[{"name":"Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry Nicolaus Copernicus University in Toru\u0144  Toru\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Ostrowska","sequence":"additional","affiliation":[{"name":"Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry Nicolaus Copernicus University in Toru\u0144  Toru\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylwia","family":"Studzi\u0144ska","sequence":"additional","affiliation":[{"name":"Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry Nicolaus Copernicus University in Toru\u0144  Toru\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.12775\/JEHS.2023.23.01.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5934"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/tnsci-2017-0001"},{"issue":"3","key":"e_1_2_10_5_1","article-title":"Gene Therapy for Spinal Muscular Atrophy (SMA): A Review of Current Challenges and Safety Considerations for Onasemnogene Abeparvovec 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D.Fraier M.Donzelli P.Dzygiel L.Ferrari andFHoffmann\u2010La \u201cOvercoming Bioanalytical Challenges During the Preclinical and Clinical Development of Risdiplam (Evrysdi\u00ae) for the Treatment of Spinal Muscular Atrophy in Children and Adults \u201d in14th EBF Open Symposium(Roche 2021) 1\u201313 https:\/\/e\u2010b\u2010f.eu\/wp\u2010content\/uploads\/2021\/12\/55.\u2010Katja\u2010Heinig\u2010F.\u2010Hoffmann\u2010La\u2010Roche.pdf."}],"container-title":["Journal of Separation 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