{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:15:22Z","timestamp":1789694122085,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T00:00:00Z","timestamp":1751155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82460820"],"award-info":[{"award-number":["82460820"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The chemical composition of\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    , a traditional Chinese medicine, is complex. Its efficacy relies on the interaction of its components, making quality evaluation based on a single chemical insufficient. This study proposes a multi\u2010dimensional feature network to enhance\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    quality assessment, focusing on effectiveness, content, traceability, and specificity. The network pharmacological analysis is employed to elucidate the underlying mechanism by which chemical components in\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    exert their therapeutic effects, specifically in terms of heat\u2010clearing, body fluid promotion, and detoxification. UHPLC fingerprinting analysis can systematically parse the overall chemical profile of\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    , and in combination with chemometric analysis, based on the screening criterion of variable importance projection value &gt; 1, nine potential differential compounds in\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    were screened out from the chemometric analysis. Subsequently, the UHPLC\u2010QTOF\u2010MS method, which features high sensitivity, high selectivity, high precision and is capable of multi\u2010stage mass spectrometry, was employed to qualitatively identify the aforementioned potential differential compounds to accurately reveal the chemical constituents of\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    . The rat serum was subjected to pharmacochemical analysis, which revealed the presence of nine prototype components that were absorbed into the bloodstream. The candidate components were identified and the scores for four dimensions were computed, leading to the construction of a multi\u2010dimensional feature network based on the \u201cspider web\u201d model. Key quality markers in\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    were identified as ellagic acid, chebulic acid, methyl gallate, and gallic acid. In conjunction with our prior research on the quality markers of\n                    <jats:italic>Chebulae Fructus<\/jats:italic>\n                    . The binding ability of quality markers in\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    and\n                    <jats:italic>Chebulae Fructus<\/jats:italic>\n                    to their corresponding core targets was verified using molecular docking. This multi\u2010dimensional approach founded on advanced analytical methods is capable of accurately identifying and validating key\n                    <jats:italic>Chebulae Fructus Immaturus<\/jats:italic>\n                    quality markers.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70199","type":"journal-article","created":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T11:47:35Z","timestamp":1752407255000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Study on Quality Markers of\n                    <i>Chebulae Fructus Immaturus<\/i>\n                    by the Integration of Network Pharmacology, UHPLC Fingerprint, and Serum Pharmacochemistry"],"prefix":"10.1002","volume":"48","author":[{"given":"Qian\u2010Qian","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing\u2010Ping","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering Northwest Minzu University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.153165"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2019.01.015"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.04.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.03.025"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.01.023"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.04.015"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155678"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202400127"},{"issue":"10","key":"e_1_2_9_10_1","first-page":"944","article-title":"The Application of Modern Analytical Techniques in the Investigations of Traditional Chinese Medicine","volume":"43","author":"Li Z.","year":"2024","journal-title":"Tianjin Journal of Traditional Chinese Medicine"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.118"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300281"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201900365"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2025.127661"},{"key":"e_1_2_9_15_1","first-page":"3009","article-title":"Screening Active Ingredients Based on Serum Pharmacochemistry of Traditional Chinese Medicine: Current Situation and Problems","volume":"45","author":"Huang C.\u2010S.","year":"2014","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202400308"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2017.12.034"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.104296"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184331"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6702"},{"key":"e_1_2_9_21_1","unstructured":"C. P. Commission Pharmacopoeia of the People's Republic of China Part OneBeijingChina Medical Science Pres(2020):135\u2013876."},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2016.04.048"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27144617"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2021.106438"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf302925v"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115847"},{"issue":"3","key":"e_1_2_9_27_1","first-page":"254","article-title":"Analysis on Chemical Constituents from Terminalia Chebula Retz. And Terminalia Bellerica (gaertn.) Roxb. By UPLC\u2010Q\u2010exactive Quadrupole\u2010orbitrap Mass Spectrometry","volume":"41","author":"Kun Z.","year":"2020","journal-title":"Journal of Chinese Mass Spectrometry Society"},{"issue":"4","key":"e_1_2_9_28_1","first-page":"607","article-title":"Multi\u2010Compound Pharmacokinetic Research on Chinese Herbal Medicines: Identifying Potentially Therapeutic Compounds and Characterizing Their Disposition and Pharmacokinetics","volume":"42","author":"Li C.","year":"2021","journal-title":"Acta Pharmaceutica Sinica B"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.03.006"},{"issue":"9","key":"e_1_2_9_30_1","first-page":"2548","article-title":"Discovery Strategy and Application of Quality Markers of Traditional Chinese Medicine Based on Component Specificity","volume":"52","author":"Liu Y. C.","year":"2021","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0633-9492"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-018-0477-z"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.152906"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24193483"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3463"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b12117"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T08:43:40Z","timestamp":1764665020000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,29]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/jssc.70199"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70199","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,29]]},"assertion":[{"value":"2025-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70199"}}