{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:06:52Z","timestamp":1789787212706,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fatty acids (FAs) are important metabolites for various biochemical functions in living organisms, including energy storage, cell structure, and cell signaling. Changes in FAs composition were significantly related to abnormal metabolic levels and body status. In this study, the derivatization\u2010based liquid chromatography\u2010quadrupole\u2010orbitrap mass spectrometry method was established for FA analysis under parallel reaction monitoring mode. The 4\u2010amino\u20101\u2010benzylpiperidine (4A1BP) was used as a derivatization reagent to amide with FAs. The results showed that protonated molecules of FA+4A1BP\u2013H\n                    <jats:sub>2<\/jats:sub>\n                    O were observed, and characteristic fragment ions at\n                    <jats:italic>m<\/jats:italic>\n                    \/\n                    <jats:italic>z<\/jats:italic>\n                    174.1280 and 91.0548 were detected in the tandem mass spectrum. The derivatization method was optimized in terms of sample preparation, chromatographic separation, and mass spectrometric conditions. The linearity, stability, and repeatability of the method were also investigated with good results. Finally, we applied the established method to analyze the serum and liver tissue samples, successfully evaluating the efficacy of Qizha Shuangye granules in the treatment of hyperlipidemia rats. The established method has broad utility and great potential in the detection of FAs, which can provide technical support for disease mechanism research, biomarker discovery, as well as food quality assurance, and nutritional value evaluation.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70211","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T13:39:39Z","timestamp":1751636379000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Chemical Derivatization\u2010Based Liquid Chromatography\u2010Mass Spectrometry Method for Fatty Acid Profiling in Biological Samples"],"prefix":"10.1002","volume":"48","author":[{"given":"Jingxuan","family":"Yang","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wu","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medicinal Materials Jilin Academy of Chinese Medicine Sciences  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Jilin Ginseng Academy Changchun University of Chinese Medicine  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2022.06.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.605154"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjdrc-2018-000632"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00098.2020"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-021-11313-y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874091X01004010053"},{"key":"e_1_2_8_8_1","first-page":"e867","article-title":"Omega\u20103 Polyunsaturated Fatty Acid (Fish Oil) Supplementation and the Prevention of Clinical Cardiovascular","volume":"135","author":"Siscovick D. S.","year":"2017","journal-title":"Disease"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0904-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11010072"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2217\/clp.14.61"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.3.562"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.10.018"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20295"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202000160"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2020.115988"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.18SCP10"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2018-0241"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664-023-00286-8"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.13213"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27175717"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.70015"},{"key":"e_1_2_8_23_1","first-page":"71","article-title":"Advances in Chemistry Research","author":"N\u00fa\u00f1ez O.","year":"2013","journal-title":"Nova Science Publishers"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.O112.019802"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-020-02813-3"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.70051"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2022.10.019"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.34799"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.339"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2017.03.005"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jjcc.2016.12.012"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-7075-8-65"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-10-18"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.1.259"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.1075421"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.05.008"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100611"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T08:43:41Z","timestamp":1764665021000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/jssc.70211"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70211","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70211"}}