{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T02:29:49Z","timestamp":1781490589695,"version":"3.54.1"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the chain length\u2010dependent extraction efficiency of two cationic surfactants\u2014dodecyltrimethylammonium bromide (C12, 12\u2010carbon chain) and cetyltrimethylammonium bromide (C16, 16\u2010carbon chain)\u2014for reverse micelle extraction of prolamins from Baijiu Jiuzao (Chinese Baijiu distillers' grains). Although pH variations (4.0\u20139.0) had negligible effects, ethanol, ionic strength, and chaotropic agent demonstrated distinct chain length\u2010dependent behaviors: ethanol increased C12's forward extraction efficiency by 10.6% (absolute) while enhancing C16's backward efficiency by 8.7%. Potassium chloride enhanced C16's forward efficiency by 4.7%, while potassium bromide significantly improved C12's backward efficiency by 15.9%. Guanidine hydrochloride consistently promoted C16 in both extraction phases. Dynamic light scattering demonstrated significant differences (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05) in reverse micelle sizes: C12 formed smaller empty reverse micelles (1.5 \u00b1 0.2\u00a0nm) compared to C16 (2.5 \u00b1 0.5\u00a0nm). During forward extraction, reverse micelles expanded significantly to 217.9 \u00b1 6.3\u00a0nm (C12) and 311.2 \u00b1 43.2\u00a0nm (C16). Backward extraction released prolamin particles of 449.0 \u00b1 16.2\u00a0nm (C12) and 650.4 \u00b1 29.7\u00a0nm (C16), confirming the size\u2010exclusion\u2010driven release mechanism. The more compact C12 reverse micelles correlated with lower total prolamin yield (26.6 \u00b1 0.1%\u00a0vs. 32.2 \u00b1 0.1% for C16,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01) and substantially diminished bovine serum albumin recovery (5.2 \u00b1 0.2%\u00a0vs. 45.3 \u00b1 2.5%,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01). Sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis analysis corroborated these findings, further demonstrating that reverse micelle\u2010extracted prolamins formed thermally stable dimers resistant to reductive cleavage. Combined with dynamic light scattering data (particle size range: 400\u2013700\u00a0nm in the backward\u2010extraction aqueous phase), these findings demonstrate that prolamin aggregates after reverse micelle extraction. These findings demonstrate that surfactant chain length as a key parameter for optimizing reverse micellar systems, where shorter\u2010chain surfactants facilitate selective extraction, whereas longer chains enhance yields.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70242","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T09:02:42Z","timestamp":1754384562000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chain Length\u2010Dependent Reverse Micelle Extraction of Prolamins From Baijiu Jiuzao: Synergistic Effects of Ethanol, Ionic Strength, and Chaotropic Agent"],"prefix":"10.1002","volume":"48","author":[{"given":"TingTing","family":"Yu","sequence":"first","affiliation":[{"name":"College of Chemical Engineering Sichuan University of Science and Engineering  Zigong 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