{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:39:04Z","timestamp":1791614344032,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The therapeutic efficacy of traditional Chinese medicines (TCMs) is intrinsically linked to their quality. Differentiating Dao\u2010di medicinal materials from non\u2010Dao\u2010di ones is essential for ensuring the authenticity, superior quality, and therapeutic efficacy of TCMs.\n                    <jats:italic>Corydalis decumbens<\/jats:italic>\n                    (Thunb.) Pers. (\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    ) is a renowned traditional Chinese medicinal herb widely employed in clinical practice for treating various diseases, including blood stasis, stroke, hemiplegia, rheumatoid arthritis, and headache. To date, few studies have addressed the geographical origin differentiation of\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    , and the specific chemical constituents responsible for regional variations remain unclear. In this study, a UHPLC\u2010HRMS (ultra\u2010high\u2010performance liquid chromatography coupled with high\u2010resolution mass spectrometry) method, in combination with chemometric tools, was developed for the comprehensive profiling of\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    collected from various geographical regions. First, an in\u2010depth characterization of\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    was performed using the established UHPLC\u2010HRMS method. By integrating database matching, fragmentation pathway elucidation, and comparison with authentic standards, a total of 151 compounds were identified, including 131 isoquinoline alkaloids. More specifically, 86 of these compounds were reported in\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    for the first time, including eight isoquinoline alkaloids bearing previously unreported substituent combinations. Based on the identified compounds, principal component analysis (PCA) and orthogonal partial least\u2010squares discriminant analysis (OPLS\u2010DA) were performed to distinguish 33 batches of\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    derived from six geographical origins. The analysis revealed that alkaloids were the primary discriminative constituents contributing to origin differentiation. This work has expanded the chemical foundation of\n                    <jats:italic>C. decumbens<\/jats:italic>\n                    and offers valuable insights into quality control and geographical origin discrimination research.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70247","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T06:15:43Z","timestamp":1755929743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comprehensive Analysis of\n                    <i>Corydalis decumbens<\/i>\n                    From Different Regions Using UHPLC\u2010HRMS and Chemometrics"],"prefix":"10.1002","volume":"48","author":[{"given":"Jinfeng","family":"Zhu","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine Ganjiang Chinese Medicine Innovation Center  Nanchang 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Innovation Center  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine Ganjiang Chinese Medicine Innovation Center  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfang","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine Ganjiang Chinese Medicine Innovation Center  Nanchang China"},{"name":"State Key Laboratory of Phytochemistry and Natural Medicines, Dalian Institute of Chemical Physics Chinese Academy of Sciences  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijin","family":"Shen","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional 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