{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:23:56Z","timestamp":1783729436523,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T00:00:00Z","timestamp":1755907200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22174117"],"award-info":[{"award-number":["22174117"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Here, we present a copper\u2010mediated magnetic molecularly imprinted polymer (Cu\u2010mMIP) as dispersive solid\u2010phase extracting material (dSPE) for fast, selective, and specific extraction of a metabolic biomarker vitamin B1 (VB1) in complex biological matrices. With emulsifier\u2010free emulsion polymerization using styrene and itaconic acid as functional co\u2010monomers, Cu\n                    <jats:sup>2+<\/jats:sup>\n                    as central atom, and Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    nanoparticles (NPs) as core, the resulted Cu\u2010mMIP addresses VB1's structural challenges (conformational flexibility, hydrophilicity) while enabling rapid magnetic separation (&lt; 10 s). The Cu\u2010mMIP demonstrates exceptional specificity for VB1, achieving an imprinting factor of 5.63 and a maximum adsorption capacity of 48.75\u00a0mg\/g, 2.36\u2010fold higher than non\u2010copper counterparts. Adsorption equilibrium is attained within 20\u00a0min, driven by chemisorption via pseudo\u2010second\u2010order kinetics. Competitive binding assays reveal twofold selectivity for VB1 over its structural analog thiamine pyrophosphate and negligible interference from other B vitamins (VB2, VB9, and VB12). When applied to simulated human plasma, the method achieved a detection limit of 9\u00a0ng\/mL (\n                    <jats:italic>S<\/jats:italic>\n                    \/\n                    <jats:italic>N<\/jats:italic>\n                    = 3) with recoveries of 84.3%\u201390.5% at three spiking levels (RSD &lt; 5%,\n                    <jats:italic>n<\/jats:italic>\n                    = 3), effectively eliminating matrix interferences. This work establishes a scalable, high\u2010efficiency platform for clinical nutrient analysis, combining molecular imprinting precision with metal coordination robustness, and advances separation science by addressing critical challenges in biomarker enrichment and high\u2010throughput sample pretreatment.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70255","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T11:51:30Z","timestamp":1755949890000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Emulsifier\u2010Free Emulsion Polymerization\u2010Based Copper\u2010Mediated Molecularly Imprinted Polymer for Vitamin B1 Extraction"],"prefix":"10.1002","volume":"48","author":[{"given":"Le","family":"Wu","sequence":"first","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziying","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzhu","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1602-0244","authenticated-orcid":false,"given":"Shun","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8192-2500","authenticated-orcid":false,"given":"Chungu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering Southwest Jiaotong University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.63.3651"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13207"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20230374"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1479164119878427"},{"key":"e_1_2_11_6_1","first-page":"54","article-title":"Strategies for the Detection and Elimination of Matrix Effects in Quantitative LC\u2010MS Analysis","volume":"32","author":"Hewavitharana A. K.","year":"2014","journal-title":"LC GC North America"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/jfca.1993.1017"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2024.126324"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9TB02839K"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.teac.2019.e00063"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2019.103314"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.70035"},{"key":"e_1_2_11_13_1","first-page":"1749","article-title":"Molecularly Imprinting Polymers for Detection and Removal of Environmental Endocrine Disruptors","volume":"31","author":"Xie X. W.","year":"2019","journal-title":"Progress in Chemistry"},{"key":"e_1_2_11_14_1","first-page":"148","article-title":"Application of Molecularly Imprinted Solid\u2010Phase Extraction for the Determination of Antibiotics Residues in Environment and Food","volume":"44","author":"Hou M.","year":"2025","journal-title":"Chinese Journal of Analysis Laboratory"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664-024-00317-y"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70161"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.03.036"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2017.02.033"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7NJ03396F"},{"key":"e_1_2_11_20_1","first-page":"59","article-title":"Research Progress on Types of Template Molecules in Molecular Imprinting","volume":"42","author":"Jing L.","year":"2022","journal-title":"Modern Chemical Industry"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CS00061D"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c04959"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2022.115023"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2022.114674"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3AY00920C"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.136921"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2023.133589"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtsust.2023.100599"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2018.09.168"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300157"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2022.131487"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201900589"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/am5020666"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.02.068"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2019.12.003"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2021.10.006"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T08:42:38Z","timestamp":1764664958000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":35,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/jssc.70255"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70255","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2025-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70255"}}