{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:21:51Z","timestamp":1791606111742,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The extraction of bioactive compounds from natural plants has received considerable attention, with deep eutectic solvents (DESs) being considered as a novel kind of green solvent in the extraction processes. To extract bioactive ingredients from natural plants, a series of DESs was synthesized by combining a newly Gemini ionic liquid (IL), C<jats:sub>3<\/jats:sub>(MIM)<jats:sub>2<\/jats:sub>Br<jats:sub>2<\/jats:sub> with ethylene glycol (EG) in various molar ratios (1:3, 1:4, 1:5, and 1:6). The structure of the C<jats:sub>3<\/jats:sub>(MIM)<jats:sub>2<\/jats:sub>Br<jats:sub>2<\/jats:sub>\/EG(1:6) system was characterized using Fourier\u2010transform infrared spectroscopy, as well as <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C nuclear magnetic resonance spectroscopy. The synthesized DESs were employed for the ultrasonic\u2010assisted extraction (UAE) of flavonoids from <jats:italic>Lycium barbarum<\/jats:italic> L., and the flavonoid was quantified using ultraviolet\u2010visible spectroscopy. Key process parameters, including extraction time, temperature, and solid\u2010liquid ratio, were optimized through single\u2010factor analysis and <jats:italic>L<\/jats:italic><jats:sub>9<\/jats:sub>(3<jats:sup>3<\/jats:sup>) orthogonal array experiments. The results demonstrated optimal extraction conditions as follows: extraction time of 40\u00a0min, extraction temperature of 50\u00b0C, and a solid\u2010liquid ratio of 1\/50 (g\/mL) (the ratio of the mass of the solid material to the volume of the liquid as the extract, g\/mL). Statistical analysis revealed that extraction temperature and extraction time had both probability values less than 0.05, indicating a significant impact on the extraction rate, and the extraction yield of flavonoids reached 17.94\u00a0mg\/g, exceeding that achieved with 60% ethanol. The Gemini IL\u2010based DESs exhibited distinct advantages in flavonoid extraction compared to traditional organic solvents. Additionally, the mechanisms underlying UAE using DESs were investigated through thermogravimetric analysis and scanning electron microscopy. The findings indicate that ultrasonically\u2010assisted incorporation of Gemini ILs holds promise for advancing the extraction of bioactive substances.<\/jats:p>","DOI":"10.1002\/jssc.70265","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T09:57:07Z","timestamp":1758103027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ultrasound\u2010assisted Extraction of Flavonoids From <i>Lycium barbarum<\/i> L. using Gemini Ionic Liquid\u2010based Deep Eutectic Solvents"],"prefix":"10.1002","volume":"48","author":[{"given":"Yingying","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Material and Chemical Engineering Zhengzhou University of Light Industry  Zhengzhou P. R. 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