{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T20:50:07Z","timestamp":1768164607364,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A novel strategy combining XAD\u20104 macroporous resin pretreatment and online\u2010storage inner\u2010recycling high\u2010speed counter\u2010current chromatography (OS\u2010IRCCC) was developed for efficient separation of multi\u2010polarity constituents from\n                    <jats:italic>Polygonatum cyrtonema<\/jats:italic>\n                    Hua. The innovation lies in the OS\u2010IRCCC mode, which integrates online storage of target fractions via a six\u2010way valve\u2010equipped recycling loop and inner\u2010recycling separation, overcoming limitations of conventional high\u2010speed counter\u2010current chromatography in resolving structurally similar compounds. After resin fractionation, 40%, 60%, and 95% ethanol eluates were separated using methyl tert\u2010butyl ether\/\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butanol\/acetonitrile\/water (1:4:1:5, v\/v) solvent system, chloroform\/methanol\/water (7:6:4, v\/v) solvent system (with OS\u2010IRCCC for Compounds\n                    <jats:bold>8<\/jats:bold>\n                    and\n                    <jats:bold>9<\/jats:bold>\n                    ), and petroleum ether\/ethyl acetate\/methanol\/water (5:5:4:3, v\/v) solvent system, respectively. Thirteen compounds were successfully isolated, including four high\u2010polarity glycosides (\n                    <jats:bold>1<\/jats:bold>\n                    \u2013\n                    <jats:bold>4<\/jats:bold>\n                    ), five medium\u2010polarity phenolic constituents (\n                    <jats:bold>5<\/jats:bold>\n                    \u2013\n                    <jats:bold>9<\/jats:bold>\n                    ), and four low\u2010polarity terpenes (\n                    <jats:bold>10<\/jats:bold>\n                    \u2013\n                    <jats:bold>13<\/jats:bold>\n                    ). This study demonstrates that the established separation method provides an efficient, rapid, and straightforward approach for isolating active constituents from\n                    <jats:italic>P. cyrtonema<\/jats:italic>\n                    Hua.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70329","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:38:47Z","timestamp":1764333527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Efficient Method to Separate the Main Constituents From\n                    <i>Polygonatum cyrtonema<\/i>\n                    Hua by a Combination of Online\u2010Storage Inner\u2010Recycling and Consecutive High\u2010Speed Counter\u2010Current Chromatography"],"prefix":"10.1002","volume":"48","author":[{"given":"Kai","family":"Zhong","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Qilu University of 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