{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T16:28:25Z","timestamp":1784046505864,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A wet oxidation method was developed to remove the bonded phase from silica\u2010based packing materials in spent chromatographic columns, thereby regenerating sub\u20103\u00a0\u00b5m monodisperse superficially porous particles (SPPs). The (2\u2010hydroxyethyl)\u2010\u03b2\u2010cyclodextrin was grafted onto the surface of the regenerated particles, creating a chiral stationary phase (CSP). For comparison, CSPs were also prepared based on commercially available fully porous particles (3 \u00b5m, FPPs) and commercially available SPPs (2.7 \u00b5m). The chiral separation performance of these CSPs was evaluated using nine racemic compounds in reversed\u2010phase liquid chromatography (RPLC) and supercritical fluid chromatography (SFC). The results showed that the regeneration method effectively removed the bonded phase from the spent column packing materials while maintaining the structural integrity of the SPP. Under the same chromatographic conditions, the SPP\u2010CSP achieved a comparable enantioselectivity relative to the FPP\u2010CSP while demonstrating superior column efficiency. The SPP\u2010CSP displayed higher enantioselectivity when chromatographic conditions were altered to yield equivalent analysis times. SFC and RPLC demonstrated complementary chiral recognition ranges. SFC achieved higher enantioselectivity and better chromatographic performance within their jointly effective separation ranges. Compared to commercially available (2\u2010hydroxypropyl)\u2010\u03b2\u2010cyclodextrin column of identical structure and specifications, the (2\u2010hydroxyethyl)\u2010\u03b2\u2010cyclodextrin SPP\u2010CSP exhibits different enantioselectivity. CSPs prepared from regenerated SPPs exhibit no significant differences in chiral separation performance compared to commercially available SPPs of the same brand, structure, and specifications. The (2\u2010hydroxyethyl)\u2010\u03b2\u2010cyclodextrin SPP\u2010CSP, synthesized via a straightforward and uncomplicated method, demonstrates favorable stability and chiral resolution capabilities.<\/jats:p>","DOI":"10.1002\/jssc.70336","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T05:05:24Z","timestamp":1766984724000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Silica Regeneration and Chiral Separation: Comparative Performance of (2\u2010Hydroxyethyl)\u2010\u03b2\u2010Cyclodextrin\u2010Grafted Superficially Porous Versus Fully Porous Particles in Reversed\u2010Phase Liquid Chromatography and Supercritical Fluid Chromatography"],"prefix":"10.1002","volume":"48","author":[{"given":"Guangyong","family":"Yang","sequence":"first","affiliation":[{"name":"Research Institute for Basic Quality Development of Xinjiang Uygur Autonomous Region  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufang","family":"Tang","sequence":"additional","affiliation":[{"name":"China Merchants Xinjiang Quality Testing Technology Research Institute Co. 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Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huangtao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute for Basic Quality Development of Xinjiang Uygur Autonomous Region  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yang","sequence":"additional","affiliation":[{"name":"Xinjiang Uygur Autonomous Region Science and Technology Resource Sharing Service Center  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Institute for Basic Quality Development of Xinjiang Uygur Autonomous Region  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3866\/PKU.DXHX201904021","article-title":"Great Concern for Chiral Pharmaceuticals From the Thalidomide Tragedy","volume":"34","author":"Zhang W.","year":"2019","journal-title":"University Chemistry"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2022.121034"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2025.343664"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.07.040"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.01.065"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2025.465727"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1061934823030048"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00081a019"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(00)00804\u20109"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530070614"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(01)00958\u2010X"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja992421q"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(97)00648\u20101"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.08.075"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)82245-1"},{"key":"e_1_2_7_17_1","first-page":"173","article-title":"Binaphthyl\u20102,2\u2032\u2010diyl Hydrogen Phosphate. 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