{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T18:50:52Z","timestamp":1769712652595,"version":"3.49.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360722"],"award-info":[{"award-number":["82360722"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study proposed a general strategy of \u201cpre\u2010column derivatization\u2010chiral selector synergy\u201d and achieved preparation\u2010level separation in the gossypol derivative system for the first time. In this study, gossypol acetate was derived by chloramphenicol base (\n                    <jats:sc>d<\/jats:sc>\n                    \u2010(\u2010)\u2010threo\u20101\u2010(p\u2010nitrophenyl)\u20102\u2010amino\u20101,3\u2010propanediol), and the derivatives were separated by high\u2010speed countercurrent chromatography using HP\u2010\u03b2\u2010CD (Hydroxypropyl\u2010\u03b2\u2010cyclodextrin) as chiral selector. According to the test results, a two\u2010phase solvent system with a volume ratio of 7:13:8:12 water: methanol: ethyl acetate: cyclohexane was selected. The factors influencing the separation process were investigated. The final conditions are 65% methanol content in aqueous phase, 60% ethyl acetate content in organic phase, HP\u2010\u03b2\u2010CD concentration was 0.05\u00a0mol\/L, pH value was 2.37, and temperature was 20\u00b0C. The purity of the enantiomers of gossypol acetate is more than 95% (it was calculated by the area normalization method), and the recoveries of gossypol derivatives are between 80% and 90%.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70352","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:50:22Z","timestamp":1768830622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preparative Enantiomer Separation of Gossypol as Chloramphenicol Derivative With Cyclodextrin as Chiral Selector by High Speed Countercurrent Chromatography"],"prefix":"10.1002","volume":"49","author":[{"given":"Zishuo","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmacy Xinjiang Medical University Urumqi China"},{"name":"Key Laboratory of Active Components and Drug Release Technology of Natural Medicines in Xinjiang Xinjiang Medical University Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xinjiang Medical University Urumqi China"},{"name":"Key Laboratory of Active Components and Drug Release Technology of Natural Medicines in Xinjiang Xinjiang Medical University Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinqin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xinjiang Medical University Urumqi China"},{"name":"Key Laboratory of Active Components and Drug Release Technology of Natural Medicines in Xinjiang Xinjiang Medical University Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xinjiang Medical University Urumqi China"},{"name":"Key Laboratory of Active Components and Drug Release Technology of Natural Medicines in Xinjiang Xinjiang Medical University Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengying","family":"Lv","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Medical College Yangzhou University Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xinjiang Medical University Urumqi China"},{"name":"Key Laboratory of Active Components and Drug Release Technology of Natural Medicines in Xinjiang Xinjiang Medical University Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Shen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Xinjiang Medical University, Department of Pharmacy Xinjiang Key Laboratory of Clinical Drug Research Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xinjiang Medical University Urumqi China"},{"name":"Key Laboratory of Active Components and Drug Release Technology of Natural Medicines in Xinjiang Xinjiang Medical University Urumqi 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