{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T23:25:36Z","timestamp":1780615536859,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In a context where environmental concerns and circular economy drive innovation, co\u2010products from the flax industry emerge as valuable lignocellulosic feedstocks. Pyrolysis of these biomasses generates bio\u2010oils with complex and highly oxygenated composition, which requires in\u2010depth molecular characterization to optimize valorization processes. This study focuses on the molecular\u2010level characterization of bio\u2010oil derived from flax shives, a local co\u2010product in Normandy. Size\u2010exclusion chromatography (SEC) was used to fractionate the bio\u2010oil into five distinct fractions based on apparent molecular size. The complete bio\u2010oil and each SEC fraction were then analyzed by high\u2010resolution mass spectrometry (Fourier\u2010transform ion cyclotron resonance [FT\u2010ICR MS] and Orbitrap MS) to identify and compare thousands of molecular formulae. A custom Python\u2010based workflow was developed to align and annotate Orbitrap MS data using the FT\u2010ICR MS dataset as a reference, allowing for rapid molecular formula assignment. Chemical class distribution, oxygen content, and Van Krevelen analysis revealed specific trends across fractions, indicating that SEC separation was influenced not only by molecular size but also by polarity and structural interactions. This approach offers a refined strategy for exploring the chemical complexity of lignocellulosic bio\u2010oils and supports future efforts in their upgrading and application.<\/jats:p>","DOI":"10.1002\/jssc.70354","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T05:47:30Z","timestamp":1769060850000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combining Fractionation and High\u2010Resolution Mass Spectrometry Approaches to Characterize a Bio\u2010Oil From Flax Shive Pyrolysis"],"prefix":"10.1002","volume":"49","author":[{"given":"Th\u00e9o","family":"Imhoff","sequence":"first","affiliation":[{"name":"INSA Rouen, INSA Rouen Normandie Univ Caen Normandie, ENSICAEN, CNRS, Institut CARMeN UMR 6064  Caen France"},{"name":"International Joint Laboratory \u2013 iC2MC: Complex Matrices Molecular Characterization, TRTG  Harfleur France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Devaux","sequence":"additional","affiliation":[{"name":"INSA Rouen, INSA Rouen Normandie Univ Caen Normandie, ENSICAEN, CNRS, Institut CARMeN UMR 6064  Caen France"},{"name":"International Joint Laboratory \u2013 iC2MC: Complex Matrices Molecular Characterization, TRTG  Harfleur France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Deschamps","sequence":"additional","affiliation":[{"name":"INSA Rouen, INSA Rouen Normandie Univ Caen Normandie, ENSICAEN, CNRS, Institut CARMeN UMR 6064  Caen France"},{"name":"International Joint Laboratory \u2013 iC2MC: Complex Matrices Molecular Characterization, TRTG  Harfleur France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Vievard","sequence":"additional","affiliation":[{"name":"INSA Rouen, INSA Rouen Normandie Univ Caen Normandie, ENSICAEN, CNRS, Institut CARMeN UMR 6064  Caen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro F.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"CESAM \u2010 Centre for Environmental and Marine Studies Department of Chemistry University of Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Afonso","sequence":"additional","affiliation":[{"name":"INSA Rouen, INSA Rouen Normandie Univ Caen Normandie, ENSICAEN, CNRS, Institut CARMeN UMR 6064  Caen France"},{"name":"International Joint Laboratory \u2013 iC2MC: Complex Matrices Molecular Characterization, TRTG  Harfleur France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina M. B. O.","family":"Duarte","sequence":"additional","affiliation":[{"name":"CESAM \u2010 Centre for Environmental and Marine Studies Department of Chemistry University of Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Mignot","sequence":"additional","affiliation":[{"name":"INSA Rouen, INSA Rouen Normandie Univ Caen Normandie, ENSICAEN, CNRS, Institut CARMeN UMR 6064  Caen France"},{"name":"International Joint Laboratory \u2013 iC2MC: Complex Matrices Molecular Characterization, TRTG  Harfleur France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"\u201cValorisation des produits et coproduits v\u00e9g\u00e9taux issus de la transition agro\u2010\u00e9cologique\u2014Analyse n\u00b0 201 \u201dMinist\u00e8re de l'Agriculture et de la Souverainet\u00e9 alimentaire(2024) https:\/\/agriculture.gouv.fr\/valorisation\u2010des\u2010produits\u2010et\u2010coproduits\u2010vegetaux\u2010issus\u2010de\u2010la\u2010transition\u2010agro\u2010ecologique\u2010analyse."},{"key":"e_1_2_9_3_1","unstructured":"\u201cEvaluation de la disponibilit\u00e9 et de l'accessibilit\u00e9 de fibres v\u00e9g\u00e9tales \u00e0 usages mat\u00e9riaux en France.\u201d Agence de l'Environnement et de la Maitrise de l'Energie(2011) https:\/\/www.bioeconomie\u2010hautsdefrance.fr\/wp\u2010content\/uploads\/2024\/08\/disponibilite\u2010et\u2010accessibilite\u2010des\u2010fibres\u2010vegetales\u2010a\u2010usages\u2010materiaux.pdf."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42247\u2010024\u201000648\u20107"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42247\u2010024\u201000806\u2010x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.7b02528"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rser.2012.04.028"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3GC41935E"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","unstructured":"J. P.Diebold A Review of the Chemical and Physical Mechanisms of the Storage Stability of Fast Pyrolysis Bio\u2010Oils Subcontractor Report No.: NREL\/SR\u2010570\u201027613 (Thermalchemie Inc. 1999).","DOI":"10.2172\/753818"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11244\u2010018\u20101009\u2010z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.energyfuels.1c02098"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201901014"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaap.2022.105694"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.energyfuels.3c02599"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.energyfuels.6b00655"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200301610"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9FD00005D"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2016.05.016"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000907"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ef0502397"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2023.463964"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.04.005"},{"issue":"38","key":"e_1_2_9_23_1","first-page":"15134","article-title":"Online Supercritical Fluid Chromatography Hyphenated to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry With Quadrupole Detection for Microalgae Bio\u2010Oil Characterization","volume":"96","author":"Devaux J.","year":"2024","journal-title":"Analytical Chemistry"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000823"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fuproc.2022.107509"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.460505"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2019.06.063"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaap.2024.106354"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202000119"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.03.020"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2019.05.016"},{"key":"e_1_2_9_32_1","unstructured":"J.Vievard \u201cPurification of Runoff Water With Co\u2010Products From the Flax Industry: Application to Heavy Metals and Polycyclic Aromatic Hydrocarbons \u201d (PhD thesis. Normandie Universit\u00e9 2024)."},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2012.06.022"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.2c00323"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","unstructured":"A. M.Striegel W. W.Yau J. J.Kirkland andD. 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