{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:27:43Z","timestamp":1792092463367,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92356303"],"award-info":[{"award-number":["92356303"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    1\u2010(9H\u2010Fluoren\u20109\u2010ylidene)\u20102,3\u2010dihydro\u20102\u2010methyl\u20101H\u2010benz[e]indene (short as FMB) as one of second\u2010generation light\u2010driven molecular motors contains a chiral carbon atom, and has attracted considerable attentions due to macroscopic chiral structures and light\u2010driven behaviors through supramolecular assembly and cooperative effects. Herein, chiral analysis and semi\u2010preparative separation of FMB enantiomers have been studied by high\u2010performance liquid chromatography (HPLC) on polysaccharide\u2010derived chiral stationary phases (CSPs). Effects of factors on chiral separation of FMB enantiomers, including type of CSP, composition and ratio of mobile phase, temperature, and flow rate, have been discussed in detail. Best separation of racemic FMB was achieved on cellulose tri(3,5\u2010dimethylphenylcarbamate)\u2010coated CSP with the resolution of 2.50 using the hexane\u2013ethanol mixture (99:1, v\/v). Then, each FMB enantiomer was obtained through semi\u2010preparative separation with enantiomeric purity over 99%, and their absolute configurations as well as elution order were determined by electronic circular dichroism spectroscopy. Surprisingly, elution reversal phenomenon of FMB enantiomers was observed on cellulose\u2010 and amylose\u2010 tri(3,5\u2010dimethylphenyl carbamate)\u2010coated CSPs, in which (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010FMB was eluted first on cellulose tri(3,5\u2010dimethylphenylcarbamate)\u2010coated CSP, and (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010enantiomer was eluted first on the other. Moreover, molecular docking simulation has been employed to explain the recognition mechanism and elution reversal of FMB enantiomers on these two CSPs. The docking results revealed that \u03c0\u2013\u03c0 stacks, \u03c0\u2013lone pair interactions, \u03c0\u2013 \u03c3, and \u03c0\u2013alkyl interactions between FMB and CSPs were the primary forces driving chiral separation, and the differences between them led to the enantiomer elution reversal on these two CSPs. Briefly, this work would provide valuable information for the separation and elution mechanisms of chiral molecular motors on polysaccharide\u2010based CSPs.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70355","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T05:35:36Z","timestamp":1769060136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["HPLC Chiral Separation and Elution Reversal Mechanism of a Typical Light\u2010Driven Molecular Motor, 1\u2010(9H\u2010Fluoren\u20109\u2010Ylidene)\u20102,3\u2010Dihydro\u20102\u2010Methyl\u20101H\u2010Benz[e]Indene"],"prefix":"10.1002","volume":"49","author":[{"given":"Wen\u2010long","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Chemistry Key Laboratory of Process Control and Quality Evaluation of Chiral Drugs Guangdong Provincial Drug Administration Guangzhou Key Laboratory of Biomedical Analytical Chemistry South China Normal University  Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010yi","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Chemistry Key Laboratory of Process Control and Quality Evaluation of Chiral Drugs Guangdong Provincial Drug Administration Guangzhou Key Laboratory of Biomedical Analytical Chemistry South China Normal University  Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Chemistry Key Laboratory of Process Control and Quality Evaluation of Chiral Drugs Guangdong Provincial Drug Administration Guangzhou Key Laboratory of Biomedical Analytical Chemistry South China Normal University  Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010jin","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chemistry Key Laboratory of Process Control and Quality Evaluation of Chiral Drugs Guangdong Provincial Drug Administration Guangzhou Key Laboratory of Biomedical Analytical Chemistry South China Normal University  Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010guang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry Key Laboratory of Process Control and Quality Evaluation of Chiral Drugs Guangdong Provincial Drug Administration Guangzhou Key Laboratory of Biomedical Analytical Chemistry South China Normal University  Guangzhou People's Republic of China"},{"name":"Guangdong Longsmall Biochemical Technology Co. Ltd  Qingyuan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"Wenzhou Institute University of Chinese Academy of Sciences  Wenzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru\u2010jian","family":"He","sequence":"additional","affiliation":[{"name":"School of Public Health Guangdong Pharmaceutical University  Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Chemistry Key Laboratory of Process Control and Quality Evaluation of Chiral Drugs Guangdong Provincial Drug Administration Guangzhou Key Laboratory of Biomedical Analytical Chemistry South China Normal University  Guangzhou People's Republic of 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