{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T23:21:50Z","timestamp":1778541710538,"version":"3.51.4"},"reference-count":66,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81603376"],"award-info":[{"award-number":["81603376"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In China, traditional Chinese medicine is often used as a dietary supplement for emergency treatment. In order meet the needs of patients, different regions have different methods. From chemistry consists to reasonable compatibilities, it has showed strong professional operation. Tianma Xingnao Capsule has been used for headache, dizziness, decreased memory, and slow reaction, yet its chemical profile remains unclear. This study innovatively integrates UHPLC\u2010Q\u2010TOF\u2010MS technology with UNIFI informatics platform and multi\u2010database (TCMSP, PubChem, ChemicalBook, ChemSpider, Chemsrc) to establish the first comprehensive chemical profile of this formulation. A total of 144 compounds were provisionally characterized based on retention time, exact mass, and mass spectra data, in conjunction with reference substances and related literature, including 26 phenylethanoid glycosides, 27 oligosaccharide esters, 22 flavonoids (15 xanthones), 12 organic acids, 11 triterpenoid saponins, 10 iridoids, 9 phenols and phenolic glycosides, 7 phenylpropanoids, 6 saccharides, 5 nucleosides, 2 amino acids, and 7 others. The proposed compound\u2010specific fragmentation pathways and establishment of a mass spectral library have made contributions to the standardization of traditional Chinese medicine. The results of this study will provide a reference for elucidating the material basis for Tianma Xingnao Capsule's therapeutic effects and quality control of its active pharmaceutical ingredients.<\/jats:p>","DOI":"10.1002\/jssc.70357","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:53:21Z","timestamp":1772981601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of Chemical Constituents in Tianma Xingnao Capsule Using Ultra\u2010High Performance Liquid Chromatography Coupled With Quadrupole Time\u2010of\u2010Flight Mass Spectrometry"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9554-3347","authenticated-orcid":false,"given":"Xin","family":"Gao","sequence":"first","affiliation":[{"name":"School of Pharmacy Health Science Center Xi'an Jiaotong University  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Health Science Center Xi'an Jiaotong University  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Health Science Center Xi'an Jiaotong University  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Health Science Center Xi'an Jiaotong University  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunzhe","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Health Science Center Xi'an Jiaotong University  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Bai","sequence":"additional","affiliation":[{"name":"Instrumental Analysis Center Xi'an Jiaotong University  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Sun","sequence":"additional","affiliation":[{"name":"Research Center Shaanxi Shengdetailin Biological Safety Technical Testing Co. Ltd  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468\u20102667(21)00249\u20108"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvy113"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.689625"},{"key":"e_1_2_10_5_1","first-page":"625","volume-title":"Pharmacopoeia of the People's Republic of China: Volume I, 2020 Edition","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"issue":"01","key":"e_1_2_10_6_1","first-page":"30","article-title":"Clinical Effect of Tianma Xingnao Capsule in Treatment of Mild or Moderate Vascular Dementia With Deficiency of Liver and Kidney and Liver Disturbance: A Randomized, Double\u2010Blind, Parallel\u2010Controlled, and Multicenter Clinical Study","volume":"40","author":"Chen W.","year":"2021","journal-title":"Journal of Anhui University of Chinese Medicine"},{"issue":"08","key":"e_1_2_10_7_1","first-page":"1202","article-title":"The Impact of Tianma Xingnao Capsule on Ischemic Reperfusion Injury in Sand Rat Brain","volume":"28","author":"Zhan H. Q.","year":"2006","journal-title":"Chinese Traditional Patent Medicine"},{"issue":"05","key":"e_1_2_10_8_1","first-page":"39","article-title":"The Impact of Tianma Xingnao Capsule on Platelet Aggregation Function","volume":"27","author":"Chen P.","year":"2006","journal-title":"Journal of Yunnan Traditional Chinese Medicine"},{"issue":"06","key":"e_1_2_10_9_1","first-page":"756","article-title":"Effects of Tianma Xingnao Capsule on Thrombosis and Coagulation System","volume":"34","author":"Yang J. Y.","year":"2009","journal-title":"Journal of Traditional Chinese Medicine"},{"key":"e_1_2_10_10_1","unstructured":"C. Y.Feng \"Clinical Observation of Tian Ma Xing Nao Capsule in the Treatment of Mildcognitive Impairment of Parkinson's Disease \" (Master's thesis Yunnan University of Chinese Medicine 2024)."},{"issue":"07","key":"e_1_2_10_11_1","first-page":"87","article-title":"Clinical Efficacy of Tianma Xingnao Capsule Combined With Oxcarbazepine in the Treatment of Primary Trigeminal Neuralgia","volume":"17","author":"Jiang M.","year":"2022","journal-title":"Chinese Pharmacoeconomics"},{"issue":"11","key":"e_1_2_10_12_1","first-page":"3478","article-title":"Identification of Chemical Components and Blood Components of Tianmaxingnao Capsules Based on UPLC\u2010Q\/TOF\u2010MS","volume":"25","author":"Sun Y. F.","year":"2023","journal-title":"Modernization of Traditional Chinese Medicine and Materia Medica\u2014World Science and Technology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202001086"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajps.2014.10.004"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2013.04.053"},{"issue":"04","key":"e_1_2_10_16_1","first-page":"566","article-title":"Analysis on Chemical Constituents in Hedera nepalensis by UPLC\/Q\u2010TOF\u2010MS\/MS","volume":"47","author":"Yang L. J.","year":"2016","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2024.106148"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202000341"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201700163"},{"issue":"01","key":"e_1_2_10_20_1","first-page":"33","article-title":"Simultaneous Determination of Nine Constituents in Tianma Xingnao Capsules by HPLC","volume":"42","author":"Shi X. R.","year":"2020","journal-title":"Chinese Traditional Patent Medicine"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2014.07.009"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300337"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70019"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70030"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e27622"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2371"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.06.011"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.12975"},{"issue":"06","key":"e_1_2_10_29_1","first-page":"817","article-title":"Identification of Chemical Constituents in Ophiocordyceps xuefengensis sp. nov. By HPLC\u2010Q\u2010TOF\u2010MS\/MS","volume":"46","author":"Zhang S. H.","year":"2015","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150647"},{"issue":"04","key":"e_1_2_10_31_1","first-page":"566","article-title":"Analysis on Chemical Constituents in Hedera nepalensis by UPLC\/Q\u2010TOF\u2010MS\/MS","volume":"47","author":"Yang L.","year":"2016","journal-title":"Chinese Traditional and Herbal Drugs"},{"issue":"05","key":"e_1_2_10_32_1","first-page":"1104","article-title":"Analysis of Chemical Constituents in Fresh, Dried and Prepared Rehmanniae Radix by UPLC\/ESI\u2010Q\u2010TOF MS","volume":"38","author":"Zhang B. Y.","year":"2016","journal-title":"Chinese Traditional Patent Medicine"},{"issue":"01","key":"e_1_2_10_33_1","first-page":"60","article-title":"Analysis of Chemical Constituents of Radix Polygalae by UPLC\/ESI\u2010Q\u2010TOF MS","volume":"35","author":"Chai S. W.","year":"2018","journal-title":"Tianjin Journal of Traditional Chinese Medicine"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.12.005"},{"issue":"05","key":"e_1_2_10_35_1","first-page":"1088","article-title":"Identification of Flavonoids in Dendrobium huoshanense and Comparison With Those in Allied Species of Dendrobium by TLC, HPLC and HPLC Coupled With Electrospray Ionization Multi\u2010Stage Tandem MS Analyses","volume":"42","author":"Liang Z. Y.","year":"2019","journal-title":"Journal of Separation Science"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010021\u201000508\u20100"},{"issue":"07","key":"e_1_2_10_37_1","first-page":"1790","article-title":"Identification of Q\u2010Markers for Cistanches Herba Based on HPLC\u2010Q\u2010TOF\u2010MS\/MS and Network Pharmacology","volume":"47","author":"Cao Z. Y.","year":"2022","journal-title":"China Journal of Chinese Materia Medica"},{"issue":"10","key":"e_1_2_10_38_1","first-page":"2658","article-title":"Identification of Chemical Constituents in Simiao Yong'an Decoction Based on UPLC\u2010LTQ\u2010Orbitrap\u2010MS","volume":"47","author":"Sun K. H.","year":"2022","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1018572"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3098"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1487903"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5621"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2023.115353"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118418"},{"issue":"12","key":"e_1_2_10_45_1","first-page":"1845","article-title":"Analysis of Composition Changes of Polygalae Radix Before and After Processing Based on UPLC\u2010LTQ\u2010Orbitrap MS","volume":"32","author":"Gao H.","year":"2021","journal-title":"Traditional Chinese Drug Research and Clinical Pharmacology"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AY01098A"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.08.095"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155808"},{"key":"e_1_2_10_49_1","first-page":"1","article-title":"Biosynthesis and Metabolic Regulation of Iridoids","author":"Zhang S. P.","year":"2024","journal-title":"Molecular Plant Breeding"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300771"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202300206"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2018.01.008"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2012.07.002"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154483"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2024.114974"},{"key":"e_1_2_10_56_1","unstructured":"J. K.Lin \"Active Constituent and Safety Study of PolygalatenuifoliaWilld \" (Master's thesis China Academy of Chinese Medical Sciences 2012)."},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201601343"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031\u20109422(00)00303\u20104"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.70090"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.03.074"},{"issue":"06","key":"e_1_2_10_61_1","first-page":"44","article-title":"Determination of Eight Components in Yuanzhi (Polygalae Radix) of Different Origins by LC\u2010MS\/MS and Its Quality Evaluation","volume":"27","author":"Sun W. T.","year":"2025","journal-title":"Journal of Liaoning University of Traditional Chinese Medicine"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2020.104759"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202200244"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.06.009"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109645"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2016.03.009"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2022.07.001"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70357","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70357","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:53:47Z","timestamp":1772985227000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":66,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/jssc.70357"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70357","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70357"}}