{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:08:00Z","timestamp":1791256080187,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    2\u2010O\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010D\u2010glucopyranosyl\u2010L\u2010ascorbic acid (AA\u20102\n                    <jats:italic>\u03b2<\/jats:italic>\n                    G), a natural ascorbic acid derivative discovered in\n                    <jats:italic>Lycium barbarum<\/jats:italic>\n                    L., has been demonstrated to exhibit multiple biological activities. This study developed an integrated approach combining high\u2010speed counter\u2010current chromatography (HSCCC) and an aqueous two\u2010phase system (ATPS) for the preparative isolation of AA\u20102\n                    <jats:italic>\u03b2<\/jats:italic>\n                    G from\n                    <jats:italic>L. barbarum<\/jats:italic>\n                    fruit. The solution system of ammonium sulfate\/ethanol\/water\/acetonitrile (19.5:28.9:53.3:2.0, w\/w) was established through systematic screening processes. To address the multi\u2010parameter optimization challenges in HSCCC, this study employed the entropy weight method to calculate the information entropy and weight coefficients of five critical parameters, thereby establishing a comprehensive evaluation methodology for HSCCC separation efficiency. Subsequently, the response surface methodology was utilized to optimize HSCCC operational conditions. Under the optimized conditions, 2.66\u00a0mg of AA\u20102\n                    <jats:italic>\u03b2<\/jats:italic>\n                    G with a purity of 91.24% was successfully isolated from a 140\u00a0mg extract. Building on the optimized HSCCC conditions, an overlapping injection mode was implemented based on the conventional single\u2010injection mode. Through cumulative purification of 700\u00a0mg extract, 10.65\u00a0mg of AA\u20102\n                    <jats:italic>\u03b2<\/jats:italic>\n                    G with an 84.53% purity was successfully obtained. Compared with the single\u2010injection mode, this mode reduced separation time by 55.68% while decreasing solvent consumption by 36.22%. Finally, the antioxidant activity of AA\u20102\n                    <jats:italic>\u03b2<\/jats:italic>\n                    G was assessed through in vitro antioxidant activity experiments. These results demonstrate the synergistic potential of the HSCCC\u2010ATPS integration as an efficient strategy for bioactive compound purification. The established protocol provides a robust method for AA\u20102\n                    <jats:italic>\u03b2<\/jats:italic>\n                    G preparation from\n                    <jats:italic>Lycium barbarum<\/jats:italic>\n                    L. fruit. Furthermore, it demonstrates the broader applicability of this hybrid technique in isolating bioactive constituents from complex biological matrices.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70359","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:23:54Z","timestamp":1769660634000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated Approach for Separation of 2\u2010O\u2010\n                    <i>\u03b2<\/i>\n                    \u2010D\u2010glucopyranosyl\u2010L\u2010ascorbic Acid From\n                    <i>Lycium barbarum<\/i>\n                    L. Fruit Using High\u2010Speed Countercurrent Chromatography Coupled With Aqueous Two\u2010Phase System"],"prefix":"10.1002","volume":"49","author":[{"given":"Yuliang","family":"Chen","sequence":"first","affiliation":[{"name":"College of Pharmacy Gansu University of Chinese Medicine  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huali","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Gansu University of Chinese Medicine  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ma","sequence":"additional","affiliation":[{"name":"Ningxia Institute of Drug Control  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Wang","sequence":"additional","affiliation":[{"name":"Gansu Pharmaceutical Group Science and Technology Research Institute  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Gansu University of Chinese Medicine  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningli","family":"Wang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory For Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Pei","sequence":"additional","affiliation":[{"name":"College of Pharmacy Gansu University of Chinese Medicine  Lanzhou China"},{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory For Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences  Lanzhou China"},{"name":"Qingdao Center of Resource Chemistry &amp; New Materials  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duolong","family":"Di","sequence":"additional","affiliation":[{"name":"College of Pharmacy Gansu University of Chinese Medicine  Lanzhou China"},{"name":"CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory For Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"19","article-title":"Integrated Multi\u2010omics Analysis Reveals Glycosylation Involving 2\u2010O\u2010\u03b2\u2010D\u2010glucopyranosyl\u2010L\u2010ascorbic Acid Biosynthesis in Lycium barbarum","volume":"26","author":"Huang J. 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