{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:56:10Z","timestamp":1772996170253,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    An integrated strategy combining ultra\u2010high pressure\u2010assisted extraction and pH\u2010zone\u2010refining counter\u2010current chromatography was developed for the efficient extraction and separation of the lower polar constituents from\n                    <jats:italic>Ganoderma lucidum<\/jats:italic>\n                    spores. The ultra\u2010high pressure extraction parameters were optimized using response surface methodology, achieving a high extraction yield of 1.739% under mild conditions (liquid\u2010to\u2010solid ratio of 21\u00a0mL\/g, pressure of 268\u00a0MPa, time of 7\u00a0min), demonstrating higher efficiency and avoidance of thermal degradation compared to conventional techniques. For separation, pH\u2010zone\u2010refining counter\u2010current chromatography was employed for the crude ultra\u2010high pressure extraction using\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane\/ethyl acetate\/methanol\/water (20:3:4:1,\n                    <jats:italic>v\/v<\/jats:italic>\n                    , 10\u00a0mM trifluoroacetic acid in the upper phase and 10\u00a0mM aqueous ammonia in the lower phase), yielding Compounds\n                    <jats:bold>1<\/jats:bold>\n                    \u2013\n                    <jats:bold>3<\/jats:bold>\n                    , Fraction I, and the eluted Fraction II. Subsequently, for Fractions I and II, pH\u2010zone\u2010refining counter\u2010current chromatography was further carried out using\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane\/ethyl acetate\/methanol\/water (6:5:4:2,\n                    <jats:italic>v\/v<\/jats:italic>\n                    , 10\u00a0mM trifluoroacetic acid in the upper phase and 10\u00a0mM aqueous ammonia in the lower phase) and\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane\/ethyl acetate\/methanol\/water (60:5:40:4,\n                    <jats:italic>v\/v<\/jats:italic>\n                    , 10\u00a0mM trifluoroacetic acid in the upper phase and 10\u00a0mM aqueous ammonia in the lower phase) as the solvent systems, yielding Compounds\n                    <jats:bold>4\u20136<\/jats:bold>\n                    and\n                    <jats:bold>7\u201310<\/jats:bold>\n                    , respectively. All these obtained 10 compounds were classified into seven fatty acids, one lipid, one terpenoid, and one phthalate ester, which were all characteristic of low polarity, especially for the fatty acids with chain lengths ranging from C\n                    <jats:sub>11<\/jats:sub>\n                    to C\n                    <jats:sub>26<\/jats:sub>\n                    . This integrated ultra\u2010high pressure extraction and pH\u2010zone\u2010refining counter\u2010current chromatography approach markedly outperforms conventional techniques by drastically shortening the extraction time and enabling a highly effective, large\u2010scale separation. Consequently, the ultra\u2010high pressure extraction and pH\u2010zone\u2010refining counter\u2010current chromatography platform represents a robust and promising methodology for the systematic investigation of hydrophobic constituents from natural resources.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70364","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:31:33Z","timestamp":1770885093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Separation of Lower Polarity Components From\n                    <i>Ganoderma lucidum<\/i>\n                    Spores by a Combined Approach of Ultra\u2010High Pressure Extraction and pH\u2010Zone\u2010Refining Counter\u2010Current Chromatography"],"prefix":"10.1002","volume":"49","author":[{"given":"Yuning","family":"Wang","sequence":"first","affiliation":[{"name":"College of Chemical Engineering Qingdao University of Science 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