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The developed HPLC method was based on an octadecylsilane\u2010bonded silica gel column (Agilent TC\u2010C18 250 mm \u00d7 4.6\u00a0mm, 5 \u00b5m) as the stationary phase, and the solvents were sodium hexafluorophosphate 0.05\u00a0mol\/L and methanol for the gradient elution at a flow rate of 1.0\u00a0mL\/min, with the injection volume of 20 \u00b5L, the ultraviolet (UV) detection at a wavelength of 210\u00a0nm, and the column temperature of 30\u00b0C. Under the established chromatographic conditions, all four target impurities were completely eluted within 30\u00a0min. The proposed method demonstrates good specificity, precision, robustness, and accuracy for the quantification of the four critical impurities in trihexyphenidyl hydrochloride, and was successfully applied for the analysis of trihexyphenidyl hydrochloride in a pharmaceutical preparation.<\/jats:p>","DOI":"10.1002\/jssc.70365","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:32:07Z","timestamp":1770885127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Validated High\u2010Performance Liquid Chromatographic Method for Simultaneous Quantification of Critical Impurities in Trihexyphenidyl Hydrochloride"],"prefix":"10.1002","volume":"49","author":[{"given":"Ying","family":"Tang","sequence":"first","affiliation":[{"name":"Hunan Key Lab of Biomedical Materials and Devices School of Biological Science and Medical Engineering Hunan University of Technology Zhuzhou 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