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This study employed ultrafiltration\u2010LC in conjunction with a deep eutectic solvent\u2010enhanced counter\u2010current chromatography method to achieve the separation and purification of three cyclooxygenase\u20102 inhibitors from\n                    <jats:italic>M. officinals<\/jats:italic>\n                    plant material. First, cyclooxygenase\u20102 inhibitors were discovered from\n                    <jats:italic>M. officinals<\/jats:italic>\n                    via ultrafiltration\u2010LC. Response surface methodology was further employed to optimize ultrasonic\u2010assisted extraction parameters. It was found the discovered cyclooxygenase\u20102 inhibitors could be significantly enriched under the optimized conditions. Using the deep eutectic solvent\u2010enhanced counter\u2010current chromatography, three target cyclooxygenase\u20102 inhibitors were successfully separated with a solvent system composed of\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane\/ethyl acetate\/DES\/water (3:4:5:2, v\/v\/v\/v). Ultimately, three cyclooxygenase\u20102 inhibitors, including 91\u00a0mg of honokiol, 41 mg of 8\u2010obovatol, 95 mg of magnolol were obtained from 500 mg sample. The prioritized magnolol was found to exhibit anti\u2010inflammatory activity via NF\u2010\u03baB pathway phosphorylation cascade activation inhibition and NF\u2010\u03baB\/p65 nuclear translocation prevention. It demonstrated the integration of ultrafiltration\u2010LC with deep eutectic solvent\u2010enhanced counter\u2010current chromatography enables the efficient separation of bioactive molecules. It not only advanced the academic understanding of the anti\u2010inflammatory active molecules of\n                    <jats:italic>M. officinalis<\/jats:italic>\n                    , but also created preconditions for the subsequent research, development, and clinical application of\n                    <jats:italic>M. officinalis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/jssc.70383","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:32:16Z","timestamp":1772980336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Deep Eutectic Solvent\u2010Enhanced Counter\u2010Current Chromatography Coupled With Ultrafiltration Liquid Chromatography for Targeted Separation of Cyclooxygenase\u20102 Inhibitors From\n                    <i>Magnolia officinalis<\/i>"],"prefix":"10.1002","volume":"49","author":[{"given":"Xiaoting","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Science Qinghai Normal University  Xining People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei Shizhen Laboratory Hubei University of Chinese Medicine  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feilong","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei Shizhen Laboratory Hubei University of Chinese Medicine  Wuhan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"He","sequence":"additional","affiliation":[{"name":"College of Pharmacy Hubei Shizhen Laboratory Hubei University of Chinese Medicine  Wuhan People's Republic of 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