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This study established a systematic strategy to enrich ginsenosides from the fibrous roots using macroporous adsorption resins and to investigate the adsorption mechanisms. Among the nine resins tested, the non\u2010polar HPD100 resin, characterized by its styrene\u2010divinylbenzene copolymer matrix and high specific surface area, exhibited superior performance, with an adsorption capacity of 194.1 \u00b1 5.8\u00a0mg\/g and desorption efficiency above 98.0 \u00b1 1.9%. Dynamic column experiments optimized the operating conditions, achieving 74.9 \u00b1 3.0% purity and 84.9 \u00b1 3.7% recovery at a flow rate of 3 bed volumes per hour, 2.3 \u00b1 0.2\u00a0mg\/mL sample concentration, and 80% ethanol eluent. The resin maintained stable performance over five adsorption\u2013desorption cycles. Kinetic and thermodynamic analyses revealed that adsorption followed a pseudo\u2010second\u2010order model and Langmuir isotherm, with spontaneous and endothermic characteristics. Structural analyses, including scanning electron microscopy, Fourier\u2010transform infrared spectroscopy, X\u2010ray diffraction, and X\u2010ray photoelectron spectroscopy, revealed hydrogen bonding, van der Waals forces, and \u03c0\u2013\u03c0 stacking as key interactions. Molecular dynamics simulations revealed a thermally enhanced binding effect, demonstrating that elevated temperatures strengthen ginsenoside\u2013resin interactions by increasing binding energy even as hydrogen bonding diminishes. This work elucidates the fundamental adsorption mechanism and establishes a theoretical basis for the high\u2010value valorization of American ginseng by\u2010products through a rationally designed, temperature\u2010controlled strategy.<\/jats:p>","DOI":"10.1002\/jssc.70384","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:53:03Z","timestamp":1773013983000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Macroporous Resin\u2013Based Adsorption Separation of Ginsenosides From American Ginseng Fibrous Roots: Process Optimization and Mechanistic Insights"],"prefix":"10.1002","volume":"49","author":[{"given":"Dandan","family":"Zhai","sequence":"first","affiliation":[{"name":"Henan Provincial Key Laboratory of Grain Resources Conservation and Utilization School of Biological Engineering Henan University of Technology  Zhengzhou China"},{"name":"Institute for Complexity Science 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