{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T07:14:52Z","timestamp":1773645292582,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1812403"],"award-info":[{"award-number":["U1812403"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n Lysionotus pauciflorus<\/jats:italic>\n Maxim. is a traditional Chinese medicinal herb with antitussive, antiasthmatic, and antioxidant activities. However, systematic studies on its quality control methods remain limited, which restricts its further development and application. In this study, an integrated strategy combining separation and analytical techniques with chemometric methods was established to screen quality markers related to the antiasthmatic activity of\n L. pauciflorus<\/jats:italic>\n . The chemical constituents of the extracts were systematically characterized using ultra\u2010high performance liquid chromatography coupled with Orbitrap high\u2010resolution mass spectrometry, and plasma pharmacochemistry was employed to investigate potential in vivo bioactive constituents. In addition, high\u2010performance liquid chromatography fingerprint profiles of samples from different batches were established. Principal component analysis and orthogonal partial least squares discriminant analysis were applied to identify key differential components. To evaluate antioxidant activity and link it with chemical constituents, radical scavenging assays combined with high\u2010performance liquid chromatography with diode array detection were conducted to identify potential antioxidant\u2010related components. Furthermore, network pharmacology and molecular docking were used to construct a compound\u2013target interaction network, from which candidate components related to antiasthmatic effects were screened. By integrating all the above results, a multidimensional \u201cspider\u2010web model\u201d was constructed, and the regression area of each component was calculated to evaluate its overall contribution. Ultimately, eight quality markers were identified: chlorogenic acid, neochlorogenic acid, nevadensin 5\u2010gentiobioside, nevadensin 5\u2010O\u2010\u03b2\u2010D\u2010glucopyranoside, forsythoside B, paraboside B, acteoside, and lysionotin.\n <\/jats:p>","DOI":"10.1002\/jssc.70387","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T06:44:06Z","timestamp":1773643446000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Ultra\u2010High Performance Liquid Chromatography\u2013High\u2010Resolution Mass Spectrometry, Chromatographic Fingerprinting, Chemometric Evaluation, and a \u201cSpider\u2010Web\u201d Model for Quality Marker Identification in\n Lysionotus pauciflorus<\/i>\n Maxim"],"prefix":"10.1002","volume":"49","author":[{"given":"Chunhua","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiali","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Shahid","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National Regional Key Technology Engineering Laboratory for Medical Ultrasound School of Biomedical Engineering Shenzhen University Medical School Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luping","family":"Tang","sequence":"additional","affiliation":[{"name":"Computer Education and Information Technology Center Guizhou Medical University Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhen","family":"Shi","sequence":"additional","affiliation":[{"name":"Pharmacy Department The Affiliated Cancer Hospital of Guizhou Medical University Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangqin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonglin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjun","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education) Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Guizhou Medical University Guian New Area China"},{"name":"School of Pharmacy Guizhou Medical University Guian New Area 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