{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T03:13:16Z","timestamp":1783221196910,"version":"3.54.6"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:00:00Z","timestamp":1772928000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204760"],"award-info":[{"award-number":["82204760"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82574689"],"award-info":[{"award-number":["82574689"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A chiral stationary phase\u2010high performance liquid chromatography\u2010tandem mass spectrometry (CSP\u2010HPLC\u2010MS\/MS) approach was developed and validated for the first time to quantify quantification of eight components: the enantiomers of three chiral components notopterol, oxypeucedanin hydrate, oxypeucedanin (in total six configurations), and achiral components nodakenin, imperatorin, isoimperatorin, bergapten, and ferulic acid, in Notopterygii Rhizoma et Radix. By adjusting the types of chiral stationary phase and the composition ratio of the mobile phase, methanol\u2013acetonitrile (75:25, v\/v) was selected as the mobile phase (flow rate 0.5\u00a0mL\/min), and three chiral components notopterol, oxypeucedanin hydrate, and oxypeucedanin were successfully separated into their enantiomers with Chiralpak IG. The method was applied to analyze both plant extracts and rat plasma samples. A considerable variation in content was observed among the eight components in the plant extracts, with their individual concentrations covering a wide range from 1.04 to 20\u00a0400 \u00b5g\/mL (see Section\u00a03 for details). Similarly, their plasma concentrations also spanned from 0.2 to 262.76\u00a0ng\/mL. The results demonstrated significant differences in the contents of the components. Notably, the chiral components exhibited marked differences between their enantiomers, suggesting that chiral components should be considered in the quality assessment and control of natural products.<\/jats:p>","DOI":"10.1002\/jssc.70390","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:52:43Z","timestamp":1773013963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Quantification of Eight Chiral and Achiral Components in Notopterygii Rhizoma et Radix Extract and Rat Plasma Based on Chiral Stationary Phase\u2010HPLC\u2010MS\/MS"],"prefix":"10.1002","volume":"49","author":[{"given":"Hongfei","family":"Wu","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luhuan","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyao","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjiao","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehan","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqi","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"State Key Laboratory of Chinese Medicine Modernization  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2023.113623"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2022.463633"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010020\u201002873\u2010w"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.14066\/j.cnki.cn21\u20101349\/r.2020.10.007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/00032719.2016.1169540"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20107799(92)90274\u2010Y"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf1008617"},{"key":"e_1_2_8_9_1","first-page":"182","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2025"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.03.022"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.13463\/j.cnki.jlzyy.2020.09.032"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1386207326666221031122803"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118589"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2021.19.034"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29143252"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114823"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20035062"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.16153\/j.1002\u20107777.2019.05.005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30030468"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.14148\/j.issn.1672\u20100482.2020.0629"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20210312.301"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2023.105758"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2902"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201053008\u20100"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2020.118176"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vibspec.2020.103181"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201800194"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202408547"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201041390\u20108"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113255"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.16438\/j.0513\u20104870.2024\u20100085"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.124332"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.125161"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.12206\/j.issn.2097\u20102024.202305039"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23020492"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23040762"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203\u2010009\u20100536\u20101"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2013.04.033"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1001\u20101528.2020.03.008"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24193574"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1001\u20101528.2024.11.033"},{"key":"e_1_2_8_42_1","doi-asserted-by":"crossref","unstructured":"X. W.Wu Y. B.Zhang L.Zhang et\u00a0al. \u201cSimultaneous Quantification of 33 Active Components in Notopterygii Rhizoma et Radix Using Ultra High Performance Liquid Chromatography With Tandem Mass Spectrometry \u201dJournal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences1092(2018):244\u2013251 https:\/\/doi.org\/10.1016\/j.jchromb.2018.06.006.","DOI":"10.1016\/j.jchromb.2018.06.006"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70390","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70390","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70390","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:52:52Z","timestamp":1773013972000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70390"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/jssc.70390"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70390","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-12-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70390"}}