{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:07:51Z","timestamp":1791950871669,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T00:00:00Z","timestamp":1774051200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","award":["2015IC001"],"award-info":[{"award-number":["2015IC001"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Amlodipine besylate, a third\u2010generation dihydropyridine calcium channel modulator, is routinely prescribed as a racemic mixture in the clinical management of arterial hypertension and ischemic chest pain. Among its enantiomeric pair, (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010amlodipine is the pharmacologically active form and is primarily responsible for the antihypertensive effect. Given that both (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010 and (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010amlodipine circulate at extremely low concentrations following oral administration, a highly sensitive analytical method is required for enantioselective monitoring. In this study, we developed a simple, sensitive, and cost\u2010effective capillary electrophoresis (CE) method for clinical application, which integrates hydroxypropyl\u2010\u03b2\u2010cyclodextrin\u2010mediated chiral recognition with acetonitrile\u2010driven field\u2010amplified sample stacking. Chiral resolution of trace amlodipine (AML) present in human plasma samples was performed in an uncoated bare fused silica capillary (effective length 31.2\u00a0cm, internal diameter 75 \u00b5m), thermostated at 25\u00b0C and operated with an imposed potential of 15\u00a0kV. The running buffer consisted of borate (6.25\u00a0mM) and phosphate (25\u00a0mM), adjusted to pH 2.5, containing 30\u00a0mg\/mL hydroxypropyl\u2010\u03b2\u2010cyclodextrin, and ultraviolet monitoring was conducted at 200\u00a0nm. This assay was fully validated for the quantitative measurement of each AML enantiomer in plasma. For (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010 and (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010amlodipine, linear calibration was achieved across the range 0.2475\u201319.80\u00a0ng\/mL and met the commonly accepted bioanalytical criteria. Comparative pharmacokinetic evaluation demonstrated no statistically significant differences between the concentration\u2013time profiles obtained with the proposed CE method and those generated by a reference liquid chromatography\u2010tandem mass spectrometry assay, confirming the accuracy and reliability of the developed procedure for routine therapeutic drug monitoring.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70394","type":"journal-article","created":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T12:24:41Z","timestamp":1774095881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hydroxypropyl\u2010\u03b2\u2010cyclodextrin\u2013assisted Acetonitrile Field\u2010amplified Stacking in Capillary Electrophoresis Enabling Ultra\u2010Trace Quantification of Amlodipine Enantiomers in Human Plasma"],"prefix":"10.1002","volume":"49","author":[{"given":"Haixia","family":"Deng","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiying","family":"Weng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorong","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Shanghai General Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"Kunming Medical University and Jointly With Enterprises and Research Institutions is Yunnan College of Modern Biomedical Industry Kunming Medical University  Kunming China"},{"name":"Department of Clinical Pharmacy Shanghai General Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,21]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jch.14709"},{"key":"e_1_2_13_3_1","volume-title":"Amlodipine","author":"Bulsara K. 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