{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T23:01:02Z","timestamp":1784329262556,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Here we present a novel, green analytical platform for the sensitive quantification of the short\u2011chain excipient monomethoxy polyethylene glycol amine, based on ultra\u2010high\u2010performance supercritical fluid chromatography\u2010tandem mass spectrometry. The core innovation is the effective coupling of supercritical carbon dioxide chromatography with the selective monitoring of protonated molecular ions ([M+H]\n                    <jats:sup>+<\/jats:sup>\n                    ), which collectively enhance sensitivity and minimize matrix interference. This method enables exceptionally rapid analysis, achieving baseline separation within 3.4 min, and demonstrates high sensitivity with a lower limit of quantification of 5\u00a0ng\/mL in rat plasma. Comprehensive validation confirmed its robustness, precision, and accuracy across the analytical range. Its environmental sustainability and practical utility were quantitatively endorsed by excellent Analytical GREEnness (0.66) and Blue Applicability Grade Index (72.5) scores. As a demonstrative application, the validated strategy was successfully employed in a rat pharmacokinetic study, establishing a fast, green, and reliable analytical framework for the safety evaluation of PEGylated compounds.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70406","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T17:08:52Z","timestamp":1776791332000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Green and Fast Ultra\u2010High Performance Supercritical Fluid Chromatography\u2010Tandem Mass Spectrometry Assay for Sensitive Quantification of Monomethoxy\u2010pentaethylene Glycol Amine in Rat Plasma: Application to Pharmacokinetic Study"],"prefix":"10.1002","volume":"49","author":[{"given":"Shuang","family":"Feng","sequence":"first","affiliation":[{"name":"Central Hospital of Dalian University of Technology Dalian University of Technology  Dalian P. R. 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